Melatonin and lycopene role on immune system against Chagas’disease.
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1
School of Pharmacy of Ribeirão Preto - University of São Paulo, Clinical, bromatological and toxicological Analysis, Brazil
Chagas’disease is an infectious illness caused by the protozoa Trypanosoma cruzi, affects over 15 million people being responsible for the high indices of morbidity and mortality. Melatonin is a pleiotropic hormone secreted by the pinealocytes displays several functions such as to regulate other hormones, keep the body's circadian rhythm, antioxidant effects and immunomodulating actions. Lycopene, a potent antioxidant and immunomodulatory carotenoid is synthesized by some commonly consumed plants such as tomatoes, but not synthesized by animals. This work aims to evaluate the influence of oral melatonin and lycopene treatment in i.p. T. cruzi infected male Wistar rats, (1 x 105 blood trypomastigotes of the Y strain). Animals were treated with melatonin (5 mg/kg) and lycopene (10mg/kg) once a day over the course of the experiment. Phenotypic and functional macrophage analysis, expression of CD11b/c marker on spleen cells and blood parasitaemia were performed. Animals were grouped in: control, infected, melatonin infected and lycopene infected. Studies were performed on 7 days after infection. Peritoneal cells were adjusted to 2×106 cells/ml, being phenotyped by means of cytofluorometric analysis. Parasites were counted by Brener’s method. Melatonin and lycopene treated animals displayed a significantly reduced number of parasites when compared to untreated counterparts. Infected and treated groups showed enhanced significant number of macrophages, as well as on its functional analyze (RT1B+) between infected treated and untreated groups. There was an increase on CD11b/c (APC marker) for treated infected groups. It seems that melatonin and lycopene contribute to reduce parasite replication and exert an immune regulating role.
Keywords:
Chagas Disease,
Melatonin,
LYCOPENE,
Immune System,
Macrophages
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Do Prado Junior
J,
Filipin
MD,
Brazao
V,
Toldo
MA,
Azevedo
AP and
Santello
F
(2013). Melatonin and lycopene role on immune system against Chagas’disease..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01116
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Received:
19 Jul 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. José Clovis Do Prado Junior, School of Pharmacy of Ribeirão Preto - University of São Paulo, Clinical, bromatological and toxicological Analysis, Ribeirão Preto, Brazil, jcprado@fcfrp.usp.br