Chitosan stimulates lymphocyte proliferation during the muscle phase of Trichinella spiralis infection in mice.
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1
University of Warsaw, Poland
Klaudia Brodaczewska, Natalia Wolaniuk, Katarzyna Donskow-Łysoniewska and Maria Doligalska
Department of Parasitology, Institute of Zoology, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Miecznikowa 1, Poland.
Chitosan is intensively studied as a potential vaccine adjuvant that can combine high efficacy with low toxicity. Yet how the polysaccharide affects immunity is unclear. Antibody titers were raised when antigen was administered with chitosan but it is not clear what mechanisms are involved. As parasitic infection down-regulates the immune response, modification of suppressed reactions may be promising for anti-parasitic protection. The aim of this study was to evaluate the effect of chitosan administration on the lymphocyte proliferative response in mice infected with T. spiralis.
C57Bl6 mice were intra-peritoneally injected with 500 μg of chitosan (> 375 kDa) dissolved in adipic acid, every day, from 5 days before till 9 days after infection with 400 L1 T. spiralis. In the muscle phase of infection, mesenteric lymph node cells were cultured without or with TCR stimulation (anti-CD3 and -CD28 antibodies); levels of proliferation and cytokines were measured.
Chitosan treatment restored cell responsiveness; they proliferated intensively and were again responsive to TCR stimulation. Also cytokine secretion was altered. Nonetheless the cells regained reactivity, the level of infection was intensively raised after chitosan treatment; over two times more muscle larvae were recovered from mice that obtained polysaccharide injections. In conclusion, although chitosan stimulated the lymphocyte response, the effect of treatment was not protective.
Acknowledgements
Research was supported by Faculty of Biology, University of Warsaw Intramural Grant (BW 501/86-102307).
Keywords:
Mesenteric lymph node,
Lymphocytes,
Trichinella spiralis,
Chitosan,
muscle phase
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Doligalska
M
(2013). Chitosan stimulates lymphocyte proliferation during the muscle phase of Trichinella spiralis infection in mice..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01117
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Received:
24 Jul 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Maria Doligalska, University of Warsaw, Warsaw, Poland, m.doligalska@biol.uw.edu.pl