Biological Evaluation of Human PBMC and Th17 cells using MILLIPLEX® MAP Cell Signaling and Cytokine Panels
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1
EMD Millipore, United States
Peripheral Blood Mononuclear Cells (PBMC) are composed of all blood cells having a round nucleus. This includes lymphocytes, monocytes, and macrophages. The lymphocyte population is made up of T cells, B cells, and Natural Killer cells. Th17 cells are recently discovered T helper cells that play important roles in the establishment and maximization of the capabilities of the immune system. Th17 cells are derived from CD4+ lymphocytes which are prevalent in human PBMC samples. In this study, we used six Milliplex® Cell Signaling panels and the Human Th17 Circulating panel to compose a biochemical profile of PBMC before, during, and after a differentiation process. We also examined the effect of Fetal Bovine Serum treatment on undifferentiated and differentiated PBMC. Our differentiation process consisted of a 7-day treatment of four cytokines, IL-1β, IL-6, IL-23, and TGFβ, as demonstrated in Zheng et al. Our results from cell signaling panels demonstrated that several analytes showed increased constitutive expression and phosphorylation after differentiation and stimulation with Fetal Bovine Serum. Additionally, the human Th17 panel showed that cytokine treatments induced significantly increased secretion of many cytokines including Th17 cell specific cytokines from human PBMCs (e.g. IL-17F). In conclusion, the Th17 and cell signaling panels will be useful tools for Th17 cell-related cytokine and signaling profiling in PBMC and various biological samples.
References
Zheng et al, Induction of Th17 Differentiation by Corneal Epithelial-Derived Cytokines, J Cell Physiol. 222(1):95-102 (2010)
Keywords:
Th17 Cells,
Cell signaling analysis,
Multianalyte Profiling,
Mutliplex Analysis,
Phosphoproteins
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune receptors and signaling
Citation:
Hwang
J,
Warmke
T,
Keith
R and
Wiese III
FW
(2013). Biological Evaluation of Human PBMC and Th17 cells using MILLIPLEX® MAP Cell Signaling and Cytokine Panels
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Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01136
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Received:
19 Jul 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Frederick W Wiese III, EMD Millipore, St Charles, United States, rick.wiese@emdmillipore.com