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Echinococcus granulosus experimental infection down-modulates Nitric Oxide production in DSS induced colitis in mice.

Lila Khelifi1*, Moussa Labsi1*, Imen Soufli1 and Chafia Touil-Boukoffa1*
  • 1 University of Sciences and Technology Houari Boumediene, Laboratory of Cellular and Molecular Biology, Algeria

Background: Inflammatory bowel diseases (IBD) are immune-mediated diseases, which are common in western industrialized countries while they are uncommon in developing countries. The hygiene hypothesis suggests that the loss of exposure to helminthes such as Echinococcus granulosus engendered a development of IBD. This parasite causes cystic hydatid disease which is endemic in Algeria. Previous data reported that helminthes reduce inflammatory process in experimental colitis. The aim of our study is to investigate the immuno-modulatory effect of the secondary infection with Echinococcus granulosus on Nitric Oxide (NO) production in Dextran Sulfate Sodium (DSS) induced colitis model. Materials and Methods: A group of Swiss mice is infected intraperitoneally (i.p) with 200 µl of a suspension containing 2000 viable protoscoleces of Echinococcus granulosus. After 3 months of infection, experimental colitis is induced in infected and uninfected mice by dissolving DSS in their drinking water for 7 days and then were sacrificed. We estimated disease activity index (DAI) which took into account body weight, stool consistency and gross bleeding. Nitric oxide (NO), is determined to investigate the inflammatory response. Results: Our results show that the disease activity index (DAI) of colitis increased at slow pace in the infected mice which developed cysts in comparison to the uninfected mice. In addition, a significant decrease in nitric oxide production is observed in plasma of infected mice. Conclusion: Our experiments suggest a potential immuno-modulatory effect of Echinococcus granulosus on Dextran Sulfate Sodium (DSS) experimental colitis by nitric oxide (NO) down-modulation.

Acknowledgements

The authors thank the technical and surgical staff of the department of thoracic surgery, Mustapha-Bacha Hospital of Algiers for providing cyst samples

Keywords: Dextran Sulfate Sodium (DSS), Echinococcus granulosus, experimental colitis, helminthes, Hygiene Hypothesis, inflammatory bowel diseases (IBD), Nitric Oxide (NO).

Conference: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014.

Presentation Type: Poster Presentation

Topic: Mucosal immunity

Citation: Khelifi L, Labsi M, Soufli I and Touil-Boukoffa C (2014). Echinococcus granulosus experimental infection down-modulates Nitric Oxide production in DSS induced colitis in mice.. Front. Immunol. Conference Abstract: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014). doi: 10.3389/conf.fimmu.2014.04.00015

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Received: 14 Sep 2014; Published Online: 01 Dec 2014.

* Correspondence:
Ms. Lila Khelifi, University of Sciences and Technology Houari Boumediene, Laboratory of Cellular and Molecular Biology, bab-ezzouar, Algiers, 16000, Algeria, freelita@yahoo.fr
Mr. Moussa Labsi, University of Sciences and Technology Houari Boumediene, Laboratory of Cellular and Molecular Biology, bab-ezzouar, Algiers, 16000, Algeria, moussalabsi@yahoo.fr
Prof. Chafia Touil-Boukoffa, University of Sciences and Technology Houari Boumediene, Laboratory of Cellular and Molecular Biology, bab-ezzouar, Algiers, 16000, Algeria, touilboukoffa@yahoo.fr