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Preventive effect of inulin on (DSS)-induced experimental colitis

Ryma Toumi1, Imene Soufli1, Hayet Rafa1 and Chafia B. Touil-Boukoffa1*
  • 1 University of Sciences and Technology Houari Boumediene, Algeria

Introduction: The inflammatory bowel diseases (IBDs), represented mainly by ulcerative colitis and Crohn disease refer to chronic and relapsing disorders that affect the gastrointestinal tract. Studies on animal models of colitis have indicated that these diseases result from a lack of tolerance to resident intestinal bacteria in genetically susceptible hosts (1,2). It seems that in IBD the mucus barrier is broken and penetrated by bacteria and inflammatory cells. The production of high levels of the nitric oxide (NO) by the inflammatory cells plays a part in tissue injury (3,4); In the current study, we investigate the potential preventive effects of inulin on dextran sulphate sodium (DSS)-induced experimental colitis in Swiss albino mice. Methods: The colitis was induced in mice via administration of 2.5% DSS in drinking water for 7 days (5). During this period the Swiss albino mice were given 1% (w/v) inulin in their drinking water ad libitum for 7 days. The production of NO was evaluated in the supernatants of peritoneal macrophages (pMϕ) cultures. The mucus production by the goblet cells in the colon was determined. Crypt damage and inflammation in the distal colon were also evaluated. Results: A significant shortening of colon length was observed and high levels of NO in pMϕ cultures were observed in DSS group compared to control. Oral administration of inulin decreased the severity of DSS-induced colitis. NO production in pMϕ supernatants was lower in inulin group and correlated with a reduction of colonic lesions. Goblet cells with Alcian blue-positive cells were visible reflecting mucus production. Conclusion: The prebiotic inulin improved DSS-induced colitis symptoms by down NO production down regulation and induction of mucus secretion suggesting an anti-inflammatory effects and a beneficial role in preventing colitis. Prebiotics seem to be promising for development of preventive strategies approach for IBD using dietary supplementation.

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Keywords: Colitis, Nitric Oxide, peritoneal macrophages, prebiotic, mucus secretion

Conference: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014.

Presentation Type: Poster Presentation

Topic: Mucosal immunity

Citation: Toumi R, Soufli I, Rafa H and Touil-Boukoffa CB (2014). Preventive effect of inulin on (DSS)-induced experimental colitis. Front. Immunol. Conference Abstract: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014). doi: 10.3389/conf.fimmu.2014.04.00017

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Received: 14 Sep 2014; Published Online: 01 Dec 2014.

* Correspondence: Prof. Chafia B Touil-Boukoffa, University of Sciences and Technology Houari Boumediene, Algiers, Algeria, touilboukoffa@yahoo.fr