Alterations in neutrophil extracellular traps release is associated with the degree of decompensation of liver cirrhosis
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1
Universidad de Guadalajara, Mexico
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2
Antiguo hospital Civil de Guadalajara "Fray Antonio Alcalde", Gastroenterology, Mexico
Background: Patients with liver cirrhosis present high susceptibility to bacterial infections. The neutrophils of these patients possess defects in the production of oxygen radicals, which are essential for the elimination of bacteria through the activation of diverse microbicidal mechanisms, such as neutrophil extracellular traps (NETs) release. Aim: Determination of NETs and neutrophil activation markers in patients with LC. Methods: We carried out neutrophil purification with Ficoll Histopaque from a sample of the peripheral blood of patients with compensated and decompensated liver cirrhosis. The neutrophils were activated with Phorbol 12-Myristate 13-Acetate to evaluate NETs release by means of fluorescence microscopy and fluorometry, while the expression of activation markers (CD69, CD80, Perforin, and CAP-18) was evaluated by flow cytometry. Results: We observed a significant diminution in NETs release capacity in terms of the degree of complication of the disease in patients with liver cirrhosis. We found no significant differences on comparing the different neutrophil activation markers; however, we observed a correlation between diminution of CD69 and CD80 expression and patients’ decompensation. Contrariwise, we observed an increase in the intracellular expression of CAP-18 and perforin. Conclusions: In this work, we demonstrated that patients with liver cirrhosis present neutrophil extracellular traps release deficiencies, which increase the risk of the establishment of complications.
Keywords:
Neutrophils,
NETs,
Liver Cirrhosis,
CD69,
LL37
Conference:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.
Presentation Type:
Poster Presentation
Topic:
Innate Immunity
Citation:
Agraz Cibrian
JM,
Segura-Ortega
JE,
Delgado-Rizo
V,
Bustos-Lopez
KS and
Fafutis-Morris
M
(2015). Alterations in neutrophil extracellular traps release is associated with the degree of decompensation of liver cirrhosis
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Front. Immunol.
Conference Abstract:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología.
doi: 10.3389/conf.fimmu.2015.05.00002
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Received:
28 Apr 2015;
Published Online:
14 Sep 2015.
*
Correspondence:
PhD. Mary Fafutis-Morris, Universidad de Guadalajara, Guadalajara, jalisco, 45116, Mexico, mfafutis@gmail.com