QUANTIFICATION AND PHENOTYPIC CHARACTERIZATION OF MONOCYTES SUBSETS IN HUMAN ATHEROSCLEROSIS
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1
State University of Campinas, Department of Clinical Pathology, Brazil
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2
State University of Campinas, Department of Clinical Medicine, Brazil
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3
Faculty of Medicine of Jundiai, Department of Morphology and Basic Pathology, Brazil
Atherosclerosis is a chronic inflammatory disease characterized by the retention of lipids and cells of the immune response in the arterial intima. Among the cells that gain access to the arterial wall, monocytes are the first to be recruited differentiating into macrophages and contributing to the formation of atheroma lesions. It has recently been reported that circulating monocytes constitute a heterogeneous population composed of three cell types, the CD14++CD16-, CD14++CD16+ and CD14+CD16++ cells, which have different functions and migratory capacity. Different populations of monocytes may be associated with the presence of risk factors for development of coronary artery disease (CAD) or even predict cardiovascular events in individuals with CAD. However, there is no consensus about the function of each monocyte subtype. Given the important role of monocytes in all stages of development of the atheroma, the objective of this study was to assess the expression of surface receptors on different populations of peripheral blood monocytes of patients with CAD. For this, we performed the quantification and phenotypic characterization of peripheral blood monocytes of patients with stable angina (SA), unstable angina (UA) and controls [with (RF) and without (C) risk factors for atherosclerosis] by flow cytometry. There were no differences in the frequency of subpopulations of monocytes among the groups, but the absolute number of total monocytes and the three subpopulations was lower in SA patients compared to the control group. The frequency of TLR2+, TLR4+ and CD36+ cells were similar among the groups. The frequency of SLAM+ cells was very low, with no difference among the groups. However, we found a lower frequency of SLAM/Tie2 double positive cells in the SA group compared to controls. The percentage of CCR2+ cells was also decreased in non-classical monocytes of SA patients. In agreement with the decreased absolute number of monocytes in peripheral blood, the lower frequency of CCR2+ and SLAN+Tie2+ cells could suggest migration to atherosclerotic lesion sites where they may contribute to the inflammatory process and angiogenesis.
Acknowledgements
Financial Support: grant 2014/00327-2 São Paulo Research Foundation (FAPESP) and CNPq.
Keywords:
Monocytes Subsets,
Atherosclerosis,
Inflammation,
Cardiovascular Diseases,
innate immunity
Conference:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.
Presentation Type:
Poster Presentation
Topic:
Innate Immunity
Citation:
Justo Junior
AS,
Villarejos
LM,
Nadruz Junior
W,
Sposito
AC,
Mamoni
RL,
De Oliveira
RT and
Blotta
MS
(2015). QUANTIFICATION AND PHENOTYPIC CHARACTERIZATION OF MONOCYTES SUBSETS IN HUMAN ATHEROSCLEROSIS.
Front. Immunol.
Conference Abstract:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología.
doi: 10.3389/conf.fimmu.2015.05.00009
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Received:
15 Apr 2015;
Published Online:
14 Sep 2015.
*
Correspondence:
Mr. Amauri S Justo Junior, State University of Campinas, Department of Clinical Pathology, Campinas, São Paulo, Brazil, justo.amauri@gmail.com