Analysis and effect of high intensity interval training program on monocytes subsets in obese individuals
Obesity-induced inflammation can be related to the development of various disorders associated with this condition, such as insulin resistance and diabetes mellitus. In obesity, monocytes migrate into the adipose tissue, where they differentiate into macrophages, contributing to the inflammatory process that takes place in obese tissues. Physical training is one of the main non-pharmacological modalities used in obesity treatment and/or prevention, and among exercise benefits is its anti-inflammatory effect. Thus, the aim of this study was to evaluate the frequency of monocytes subsets in sensitive or insulin resistant obese individuals and evaluate the effect of a high intensity interval training (HIIT) program on monocytes subsets frequency. Sixteen obese subjects were divided into two groups according to the presence (HOMA-IR ≥ 2.71) or absence of insulin resistance: 1- insulin sensitive obese (OB, n = 8; 32 ± 11 years, BMI 35 ± 4 kg/m2, HOMA-IR 1.7 ± 0.5 mmol.μU/L ², fat percentage 42 ± 8%); 2- insulin-resistant obese (OBR, n = 8; 26 ± 4 years, BMI 38 ± 5 kg/m2, HOMA-IR 5.2 ± 2.4 mmol.μU/L ², fat percentage 44 ± 8%). For pre-exercise analysis obese volunteers were matched to healthy eutrophic individuals (control, n = 8; 31 ± 10 years, BMI 21 ± 2 kg/m2, HOMA-IR 1.1 ± 0.5 mmol.μU/L ², fat percentage 29.7 ± 7.8%). OB and OBR groups were submitted to 8 weeks of HIIT, performed 3 times/week, (stimulus 8 to 12; 80 to 110% of maximum power). Blood samples were collected before the start of training and 72 hours after the last training session. The frequency of monocytes subsets (classical CD14++CD16-, intermediate CD14++CD16+, and nonclassical CD14+CD16++) was determined by flow cytometry (BD FACSCanto II). Before HIIT, both OB and OBR showed a higher frequency of non-classical monocytes (6.3 ± 3.4% and 7.2 ± 5.7% OB and OBR, respectively) compared to control eutrophic individuals (2.4 ± 2.1%) (p = 0.01). No difference on the frequency of classic (p = 0.23) and intermediate monocytes (p = 0.74) was observed between groups. There was no HIIT effect on the frequency of intermediate (p = 0.30), classical (p = 0.62) or non-classical (p = 0.43) monocytes. We conclude that obesity, by itself, leads to increase in the frequency of non-classical monocytes. High intensity interval training, during 8 weeks, did not alter monocytes subsets frequency in obese individuals.
Acknowledgements
Financial support: Fapemig (CDS APQ-01621-10), Capes (PNPD - 2455/2011) e CNPq (477154/2011-5).
Keywords:
Obesity,
Insulin Resistance,
Monocytes,
Exercise,
physical training
Conference:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.
Presentation Type:
Poster Presentation
Topic:
Innate Immunity
Citation:
Matos
MA,
Pinhal
K,
Lopes
J,
Garcia
B,
Fernandes
V,
Magalhães
F,
Amorim
F and
Rocha-Vieira
E
(2015). Analysis and effect of high intensity interval training program on monocytes subsets in obese individuals.
Front. Immunol.
Conference Abstract:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología.
doi: 10.3389/conf.fimmu.2015.05.00010
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Received:
11 May 2015;
Published Online:
14 Sep 2015.
*
Correspondence:
Ms. Mariana A Matos, UFVJM, Diamantina, Brazil, marianafisio1@yahoo.com.br