Dendritic cells internalize the foot-and-mouth disease virus involving LC3 autophagy and lysosomal LAMP proteins.
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1
Instituto Nacional de Tecnologia Agropecuaria, Instituto de Virologia, Argentina
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2
Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
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3
Laboratorio de Inmunología. Instituto de Medicina Experimental (IMEX)-CONICET, Academia Nacional de Medicina, Argentina
Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. This pathology is caused by foot-and-mouth disease virus (FMDV). Over time, the development of vaccines to prevent the spread of this illness became essential. Vaccines currently used contain the inactivated form of the virus (iFMDV). However, vaccination generates an immune response different to that induced by the infection.
It was extensile demonstrated that FMDV interacts with autophagyc LC3 protein in certain cellular lines. However, the mechanisms involved on dendritic cells (DCs) entry are not well defined. We analyzed this fact on murine DCs. In the first place, the internalization of FMDV and iFMDV (moi:10) were confirmed by confocal microscopy. Then, we evaluated the expression of LC3 by western blot, and the expression of LC3 protein was increased in FMDV-infected cells, which was corroborated by flow cytometry (3-5% vs iFMDV; CT, p<0.5). Furthermore, we studied the proteins related lysosomes Lamp-1 and Lamp-2. Both Lamp-1 and Lamp-2 were significantly increased in DCs infected with FMDV. Notably, iFMDV was not associated to any of both lysosomal membranes. Interestingly, when we assessed late lysosomal vacuoles by confocal microscopy, we showed an important colocalization of FMDV with Lamp-2.
As described, infectious virus in DCs was also associated to autophagy LC3 protein, and was found in lysosomal compartment; on the contrary, iFMDV was not associated with mature lysosomes.
Acknowledgements
The authors wish Dr. Osvaldo Zabal and Mrs. Teresa Morán for the valuable assistance with cell cultures, to Lic. Fatima Torales and Ms. Pamela Angelletti for technical assistance
This work was supported by grants from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto Nacional de Tecnología Agropecuaria and Agencia Nacional de Promoción Científica y Tecnológica, Argentina.
Keywords:
Foot-and-Mouth Disease Virus,
Dendritic Cells,
LC3 autophagy,
lysosomal LAMP proteins,
lysosomal compartment
Conference:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.
Presentation Type:
Poster Presentation
Topic:
Innate Immunity
Citation:
Langellotti
C,
Alcain
J,
Soria
I,
Gammella
M,
Quattrocchi
V,
Zamorano
P and
Vermeulen
EM
(2015). Dendritic cells internalize the foot-and-mouth disease virus involving LC3 autophagy and lysosomal LAMP proteins..
Front. Immunol.
Conference Abstract:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología.
doi: 10.3389/conf.fimmu.2015.05.00037
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Received:
29 May 2015;
Published Online:
14 Sep 2015.
*
Correspondence:
Dr. Cecilia Langellotti, Instituto Nacional de Tecnologia Agropecuaria, Instituto de Virologia, Buenos Aires, Hurlingham, Buenos Aires, 1686, Argentina, langellotti.cecilia@inta.gob.ar