Systemic Salmonella infection requires Interleukin 10 production in mice
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1
Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Chile
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2
Millennium Institute on Immunology and Immunotherapy, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andrés Bello, Chile
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3
Departamento de Gastroenterología, Facultad de Medicina, Pontificia Universidad Católica de Chile, Chile
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4
Departamento de Reumatología, Facultad de Medicina, Pontificia Universidad Católica de Chile, Chile
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5
INSERM, UMR 1064, Nantes, F44093 France., France
Salmonella enterica serovar Typhimurium (S. Typhimurium) is a Gram-negative and facultative anaerobic bacterium that causes a systemic disease in several hosts. Pathogen associated molecular patterns (PAMPs) in the surface of this bacterium activate innate immune response, which initially controls and restricts infection. However, S. Typhimurium is able to disseminate from the intestine to deeper tissues and prevent T cell activation. However, the contribution of anti-inflammatory molecules to the evasion of host immune response and pathology during early stages of S. Typhimurium infection remains elusive. In this study, we have evaluated whether the anti-inflammatory cytokine interleukin-10 (IL-10) could contribute to the pathogenesis of S. Typhimurium. To assess the role of IL-10 in vivo, we performed S. Typhimurium infections in mice lacking IL-10 (IL-10-/- mice). We found that IL-10-/- mice displayed increased survival as compared to wild-type mice after infection with S. Typhimurium. Further, IL-10-/- mice had significantly reduced bacterial loads in both the spleen and the liver. We show that the intracellular survival of this pathogen was reduced in bone marrow-derived macrophages (BMMs) but not in DCs from IL-10-/- mice. Moreover, IL-10-/- DCs and BBMs produced increased levels of pro-inflammatory cytokines after S. Typhimurium infection. These findings support the notion that IL-10 works as an essential molecule to allow S. Typhimurium infection by reducing the soluble inflammatory determinants that can activate both innate and adaptive immune responses that restrict S. Typhimurium dissemination.
Acknowledgements
This study was supported by grants Fondo Nacional de Ciencia y Tecnología de Chile (FONDECYT) # 1100971, 1110604, 1140010, 1110397, 1030996, 1131012, Millennium Institute on Immunology and Immunotherapy P09/016-F and Grant “Nouvelles Equipes-nouvelles thématiques” from the La Région Pays De La Loire. CPR is supported by the Comisión Nacional de Investigación Científica y Tecnológica (CONICYT). AMK is a Chaire De La Région Pays De La Loire, Chercheur Étranger D'excellence, France.
Keywords:
Salmonella enterica serovar Typhimurium,
Interleukin-10,
Dendritic Cells,
Macrophages,
IL-10 deficient mice,
Pro-inflammatory cytokines
Conference:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.
Presentation Type:
Poster Presentation
Topic:
Infectious and parasitic diseases
Citation:
Salazar
GA,
Pizarro
DP,
Pardo
C,
Salazar
FJ,
Riedel
CA,
Álvarez
M,
Kalergis
AM and
Bueno
S
(2015). Systemic Salmonella infection requires Interleukin 10 production in mice
.
Front. Immunol.
Conference Abstract:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología.
doi: 10.3389/conf.fimmu.2015.05.00144
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Received:
12 May 2015;
Published Online:
14 Sep 2015.
*
Correspondence:
Miss. Geraldyne A Salazar, Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile, geraldyne.salazar@gmail.com