COMPARISON OF TECHNIQUES FOR DIAGNOSIS OF PULMONARY TUBERCULOSIS IN HIV/AIDS PATIENTS IN SANTA CLARA AND SIMON BOLIVAR HOSPITALS FROM BOGOTA (COLOMBIA)
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1
Universidad Colegio Mayor de Cundinamarca, Colombia
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2
Universidad Nacional, Facultad de Medicina, Colombia
According to OMS, tuberculosis is one of the most prevalent diseases worldwide because one third of the world population is infected. Over the past decades the scientists have been developing new techniques for an early diagnosis of this disease. The conventional methodologies have reduced specificity and sensitivity in patients with paucibacillary TB, especially in patients with HIV/AIDS. In this research, we did for the first time in Colombia the diagnosis of pulmonary tuberculosis in 50 patients HIV/AIDS who had respiratory disease and CD4+ T cells less than 200cells/mL in Santa Clara and Simon Bolivar hospitals from Bogota (Colombia). We compared the sensitivity and specificity of two techniques: first was smear by Ziehl-Neelsen and fluorescence staining auramine and the second one was the performance of liquid culture MGIT and solid culture Lowestein-Jensen; additionally, to all cultures with positive growth, we detected Ag MPT64 by the rapid test SD Bioline. Among samples from 50 patients, 16% had smear positive with Ziehl-Neelsen and 30% with auramine, the sensitivity was 35% and 65% respectively. Also 44% of the cultures had positive growth in Lowestein-Jensen and 46% in MGIT, the sensitivity was 96% and 100% respectively. The presence of tuberculosis complex was 15 in Lowstein-Jensen and 13 in MGIT, according to the results of SD Bioline rapid test Ag MPT64.
Keywords:
Tuberculosis, Pulmonary,
HIV/AIDS clinical studies,
Diagnosis, Differential,
Laboratories,
Technique Selection
Conference:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.
Presentation Type:
Poster Presentation
Topic:
Infectious and parasitic diseases
Citation:
Avila Castañeda
AC,
Cocuy Sanchez
PA,
Moya Molina
A,
Parra Sepulveda
J,
NAVARRETE JIMENEZ
ML and
Murcia Aranguren
MI
(2015). COMPARISON OF TECHNIQUES FOR DIAGNOSIS OF PULMONARY TUBERCULOSIS IN HIV/AIDS PATIENTS IN SANTA CLARA AND SIMON BOLIVAR HOSPITALS FROM BOGOTA (COLOMBIA).
Front. Immunol.
Conference Abstract:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología.
doi: 10.3389/conf.fimmu.2015.05.00150
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Received:
29 May 2015;
Published Online:
14 Sep 2015.
*
Correspondence:
Miss. Andrea C Avila Castañeda, Universidad Colegio Mayor de Cundinamarca, Bogotá, Colombia, andreaca6466@hotmail.com
Miss. Paola A Cocuy Sanchez, Universidad Colegio Mayor de Cundinamarca, Bogotá, Colombia, paoaleja1208@hotmail.com
Dr. MYRIAM L NAVARRETE JIMENEZ, Universidad Nacional, Facultad de Medicina, Bogotá, Colombia, mlnavarretej@unal.edu.co
PhD. Martha I Murcia Aranguren, Universidad Nacional, Facultad de Medicina, Bogotá, Colombia, mimurciaa@unal.edu.co