Effect of TNF-α, IL-β, IL4 and TGF-β cytokines by Tuberculosis Systemic on blood-brain barrier and Neural disorders in Murine Experimental Model "
Citlaltepetl
Salinas Lara1*,
Karen
Cartillon Benavides2,
Wendy
A.
Alvarado Híjar1,
Carlos
Sánchez Garibay1,
Omar
J.
Castillon Benavides3,
Miguel
M.
Alejandro Guizar4,
Manuel
A.
Flores Barrada4,
José
L.
Soto5,
Paloma
Campa Arvizu1,
Martha
L.
Tena Suck1,
Rogelio
Hernandez Pando6,
María
E.
Hernández Campos7,
Luis
A.
Jimenez Zamudio8,
Daniel
Rembao Bojórquez1 and
Fernando
Montaño Rendón1
-
1
Instituto Nacional de Neurología y Neurocirugía, Mexico
-
2
Hospital Materno Infantil DEL ISSEMYM, Mexico
-
3
Hospital Regional 1o. de Octubre, Mexico
-
4
Universisdad Juarez Autonoma de Tabasco, Mexico
-
5
INSTITUTO NACIONAL DE NEUROLOGÍA Y NEUROCIRUGÍA, Neuroinfectología, Mexico
-
6
INSTITUTO NACIONAL DE CIENCIAS MÉDICAS Y NUTRICIÓN SALVADOR ZUBIRÁN, Patología Experimental, Mexico
-
7
Escuela Superior de Medicina del Instituto Politecnico Nacional, Maestría en Ciencias de la Salud y Doctorado en investigación en Medicina, Mexico
-
8
Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Naciona, Posgrado en Ciencias Químico Biológicas., Mexico
Introduction: The mechanisms by which M. tuberculosis damages the central nervous system (CNF) and produces changes in the blood-brain-barrier (BBB) and/or in the blood-CSF barrier is not well understood yet. These changes could be dependent on the specific strain as well as on the disease´s progression time. Material and methods: In this study a strain of M. tuberculosis (LAM3) was isolated from a patient with meningitis, subsequently, BALB/c male mice were infected though the trachea with H37Rv and LAM3 strains. These mice were later on sacrificed at different days following infection (1, 3, 7, 14, 21, 28, 60, 120). Mice brains were obtained and stained with hematoxylin and eosin, argentic stain method of del Rio-Hortega, electron microscopy was used for BBB and CFS-BB, Ziehl-Neelsen and PCR and immunohistochemestry was used for citoquine detection (IL1-β, TNF-α, IFN-γ, TGF-β, IL-4). Results: Neuronal alterations were observed in the frontal cortex and the hippocampus with H37Rv and LAM3 (cell death, disorganization and eosinophilic degeneration). There were no CFU growth in the brain for neither strain, though PCR was positive for LAM3. The citoquines were positive in ependymal cells, endothelial cells and in choroid plexuses for both strains: IL-1β, TNF-α, IL-4 y TGF-B. LAM3 expressed IFN-γ with greater intensity. Structural abnormalities (cytoplasmic vacuolation, intercellular separation and necrosis) were shown at the Blood-CSF barrier for both LAM3 and H37Rv. Conclusion: Concluding by these findings that neuronal abnormalities in the frontal cortex, the hippocampus and the Blood-CSF barrier are present in post-infection with M tuberculosis, these findings being more severe in the LAM3 strain than in H37Rv and possibly mediated by citoquine presence.
Keywords:
mouse models,
TNF-α,
IL-β,
Tuberculosis Systemic,
blood-brain barrier disruption
Conference:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.
Presentation Type:
Poster Presentation
Topic:
Infectious and parasitic diseases
Citation:
Salinas Lara
C,
Cartillon Benavides
K,
Alvarado Híjar
WA,
Sánchez Garibay
C,
Castillon Benavides
OJ,
Alejandro Guizar
MM,
Flores Barrada
MA,
Soto
JL,
Campa Arvizu
P,
Tena Suck
ML,
Hernandez Pando
R,
Hernández Campos
ME,
Jimenez Zamudio
LA,
Rembao Bojórquez
D and
Montaño Rendón
F
(2015). Effect of TNF-α, IL-β, IL4 and TGF-β cytokines by Tuberculosis Systemic on blood-brain barrier and Neural disorders in Murine Experimental Model ".
Front. Immunol.
Conference Abstract:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología.
doi: 10.3389/conf.fimmu.2015.05.00154
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Received:
28 May 2015;
Published Online:
14 Sep 2015.
*
Correspondence:
Dr. Citlaltepetl Salinas Lara, Instituto Nacional de Neurología y Neurocirugía, México, 14269, Mexico, cisala69@hotmail.com