Effect of the 60 kDa heat shock protein of Klebsiella pneumoniae in murine model of collagen induced arthritis.
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1
Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas. Departamento de Inmunología, Mexico
The murine model of collagen-induced arthritis (CIA) is an experimental autoimmune disease widely used in rheumatoid arthritis (RA) models. CIA is induced in susceptible strains of rodents (rat and mouse) and nonhuman primates by immunization with collagen type II (CII), the major proteic constituent in articular cartilage. Animals develop autoimmune polyarthritis, which is characterized by cartilage destruction and bone resorption similar to RA. Involvement of heat shock proteins (HSP) in the pathogeny of autoimmune diseases has been studied in animal models of arthritis induced by Freund’s complete adjuvant (FCA), in which prior immunization of the animals with mycobacterial HSP60 was shown to protect against FCA-mediated induction of the disease. We studied the immunomodulatory effect of the 60-kDa heat shock protein of Klebsiella pneumoniae (HSP60Kp) in CII-induced arthritis. To this end, 10-week-old female Wistar rats were used, arthritis was induced by intradermal immunization at the base of the tail with collagen type II. Results showed inflammatory process and arthritis development in rat hind legs as well as abundant mononuclear cell (MN) infiltration in thin sections of the articulation. To evaluate the immunomodulatory effect of HSP60Kp, rats were immunized with the protein prior to CIA induction. A
significant decrease of the inflammatory process in hind leg articulations was observed compared to hind legs of rats with arthritis, while reduced MN cell infiltration was found in articular tissue. These results are consistent with the quantification of anti-HSP60Kp and anti-CII antibodies, as well as pro-inflammatory (IL-1α, TNF-α, IL-17) and anti-inflammatory cytokines (IL-10) in serum of all test groups. A significant increase in pro-inflammatory cytokines (IL-1α, TNF-α, IL-17) was found in the arthritis group compared to control groups. No change was observed in IL-10 levels. The effect of HSP60Kp on CIA was observed as a significant reduction in pro-inflammatory cytokines (IL-1α, TNF-α, IL-17) in HSP60Kp-immunized rats. Furthermore, the anti-inflammatory cytokine IL-10 was increased by comparison to control groups. Reduction of the inflammatory process and decreased levels of pro-inflammatory cytokines along with increased levels of anti-inflammatory cytokines suggests that HSP60Kp elicits an immunomodulatory effect on CIA. These results are relevant since this is the first time this effect has been observed with an enterobacterial heat shock protein.
Keywords:
HSP60Kp,
Immunomodulation,
Collagen induced arthritis,
murine model,
Interleukins
Conference:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.
Presentation Type:
Poster Presentation
Topic:
Autoimmunity
Citation:
Ortíz-Ortíz
MV,
Carnalla-Cortés
M,
Téllez-García
CL,
Méndez-Jurado
FI,
García-Latorre
E and
Domínguez-López
M
(2015). Effect of the 60 kDa heat shock protein of Klebsiella pneumoniae in murine model of collagen induced arthritis..
Front. Immunol.
Conference Abstract:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología.
doi: 10.3389/conf.fimmu.2015.05.00219
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Received:
22 May 2015;
Published Online:
14 Sep 2015.
*
Correspondence:
Dr. María Lilia Domínguez-López, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas. Departamento de Inmunología, México, DF, DF, 02060, Mexico, ldmguez@yahoo.com.mx