Novel dendrimeric peptide: Immune response and protection against Foot-and-mouth disease virus (FMDV) induced by synthetic peptides in cattle
Ivana
Soria1, 2,
Valeria
Quattrocchi1,
Cecilia
Langellotti1, 2,
Mariela
Gammella1,
Victoria
Gnazzo2,
Erica
Pereyra2,
Sebastian
Di Giacomo1,
David
Andreu3,
Francisco
Sobrino4,
Esther
Blanco5 and
Patricia
Zamorano1, 2*
-
1
Instituto Nacional de Tecnologia Agropecuaria, Instituto de Virologia, Argentina
-
2
Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
-
3
Universitat Pompeu Fabra, Spain
-
4
Consejo Superior de Investigaciones Científicas, Spain
-
5
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, INIA, Spain
Foot-and-Mouth disease virus (FMDV) produces a highly infectious systemic vesicular disease and affects cloven hooved animal. Foot-and-Mouth (FMD) control in endemic area is performed by regular vaccination based on inactivated whole-virus. There are many problems with this kind of vaccine: i) possible incomplete inactivation of virus, ii) production in a biosafety level 4 (OIE) laboratory in FMDV-free countries, iii) need of a cold chain to virus stability, iiii) the lack of protection against serotypes and subtypes that are not include in vaccine, and the difficulty differentiating between vaccinated and infected animals.
Dendrimeric synthetic peptides FMDV vaccines are the most promising candidates as they are highly pure, defined, stable and safe. An effective peptide vaccine needs a B-cell epitope to elicit a high neutralizing antibody response and a T-cell epitope to provide adequate cooperation between T cells and the recent mentioned B lymphocytes.
A dendrimeric peptide containing a T epitope [3A (21-35)], and four or two copies of a B epitope [site A; VP1 (136-154)] of serotype O partially protected cattle.
The aim of the study was to evaluate the immunogenicity and protective response induced by B2T and B4T replacing the T epitope sequence for the one reported as immunodominant in cattle.
Calves without antibodies against FMDV were inoculated with B2T (n=5) or B4T (n=5) by intramuscular injection with 2 mg of peptide emulsified with comercial oil adjuvant at days 0 and 21. At 37dpv a booster with 0,4mg of peptide was administered. One animal was unvaccinated.
The level of IgG against to FMDV in the B4T group were (3,7 ± 0,3) at day 44dpv, whereas in the B2T group were (3,8 ± 0,1) at the same day. The predominant isotype induce was IgG1 and there was no significant difference in antibody avidity between groups. The level of α-B4T antibodies were (4,5 ± 0,1) for the B4T group, whereas in the B2T group were (4,6 ± 0,2) at 44dpv. Lastly, mean virus neutralization titer were (1,7 ± 0.3) in B4T group and slightly lower in B2T group (1,39 ± 0,02), nevertheless, both titres were considered positive (Manual of diagnostic test and vaccines for terrestrial animals, OIE).
In general, we observed positive proliferative response of PBMC when the stimulation was with peptides, but, in these experimental conditions, the inactive virus was able to induce positive response in some of vaccinated animal. IFN-γ was secreted when cells were stimulated with virus or peptides.
At 46dpv cattle were challenged with 104DIRL of FMDV by nasal instillation and the challenge was performed in the BSL-4 OIE boxes. Then, animals were monitored for 7 days to detect the appearance of the characteristic symptoms of the disease. All vaccinated animals were protected as none of them presented foot lesions. Unvaccinated animal showed all symptoms of diseases including foot lesion at 3dpi.
Acknowledgements
The authors wish Dr. Osvaldo Zabal and Mrs. Teresa Morán for the valuable assistance with cell cultures and to MD L. Vagnoni and V. Maldonado for care the cattle.
This work was supported by the agreement Instituto Nacional de Tecnologia Agropecuar-INIA
Keywords:
dendrimeric peptide,
Foot-and-Mouth Disease Virus,
Vaccine,
Cattle,
protection
Conference:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.
Presentation Type:
Oral Presentation
Topic:
Veterinary and Comparative Immunology
Citation:
Soria
I,
Quattrocchi
V,
Langellotti
C,
Gammella
M,
Gnazzo
V,
Pereyra
E,
Di Giacomo
S,
Andreu
D,
Sobrino
F,
Blanco
E and
Zamorano
P
(2015). Novel dendrimeric peptide: Immune response and protection against Foot-and-mouth disease virus (FMDV) induced by synthetic peptides in cattle.
Front. Immunol.
Conference Abstract:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología.
doi: 10.3389/conf.fimmu.2015.05.00237
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
29 May 2015;
Published Online:
14 Sep 2015.
*
Correspondence:
Dr. Patricia Zamorano, Instituto Nacional de Tecnologia Agropecuaria, Instituto de Virologia, Buenos Aires, Hurlingham, Buenos Aires, 1686, Argentina, zamorano.patricia@inta.gob.ar