Interleukin (IL)-17AA Reduces Pro-Inflammatory Cytokine Production By Inflammatory Bowel Disease Mucosa Cultured Ex Vivo
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1
CONICET-CCT La Plata- UNLP, Instituto de Inmunología y Fisiopatología, Argentina
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2
Queen Mary University of London, Barts and The London School of Medicine and Dentistry, United Kingdom
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3
Hospital de Gastroenterología Bonorino Udaondo, Argentina
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4
Hospital Interzonal General de Agudos "Gral. San Martín", Servicio de Gastroenterología, Argentina
Inflammatory bowel disease (IBD) are chronic disorders in which pro-inflammatory cytokines play a key role in the immunopathogenesis. Interleukin (IL)-17A was found to be up-regulated in mucosal lesions from IBD patients. IL-17 cytokines family includes 5 members (IL-17A/F/E/C and D), which constitute dimers in their active form. IL-17A shares 50% homology with IL-17F, they may form IL-17AA and IL-17FF homodimers or IL-17A/F heterodimers. Since the role of IL-17 dimers is unknown in IBD, we studied the pro-inflammatory effect of IL-17AA, IL-17FF and IL-17-A/F in intestinal samples of ulcerative colitis (UC) and Crohn’s disease (CD) patients.
Inflamed colonic biopsies from 26 IBD patients (13 UC and 13 CD) were cultured ex vivo for 24 hours with IL-17AA, IL-17FF or IL-17A/F (1 ng/ml). Mucosal myofibroblasts isolated from the inflamed colon of 4 CD and 4 UC patients were cultured with increasing concentrations (1-100 ng/ml) of each dimer or tumor necrosis factor (TNF)-alpha (20 ng/ml) as control. IL-6 and IL-8 were measured in culture supernatants by ELISA.
We found in inflamed IBD biopsies that IL-17AA, but not IL-17FF, significantly reduced both IL-6 and IL-8 production, whereas IL-17A/F only decreased IL-8 release. As expected, TNF-alpha stimulation significantly increased IL-6 and IL-8 production in CD and UC myofibroblasts. However, neither IL-17AA, nor IL-17FF, nor IL-17A/F modified the secretion of IL-6 and IL-8 in IBD myofibroblasts at these tested concentrations.
In conclusion, we found that IL-17AA exerted an anti-inflammatory action on inflamed IBD biopsies, and this effect would not be directly mediated by myofibroblasts. Further studies are needed to identify the cell target for IL-17AA in IBD mucosa.
Keywords:
Il-17 cytokines,
organ culture assay,
mucosal explants,
Chron's disease,
ulcerative colitis,
Inflamatory bowel disease
Conference:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.
Presentation Type:
Poster Presentation
Topic:
Mucosal Immunity and the microbiome
Citation:
Curciarello
R,
Biancheri
P,
Rocca
A,
Sambuelli
A,
Correa
G,
Yantorno
M,
Chopita
N,
MacDonald
TT and
Docena
GH
(2015). Interleukin (IL)-17AA Reduces Pro-Inflammatory Cytokine Production By Inflammatory Bowel Disease Mucosa Cultured Ex Vivo.
Front. Immunol.
Conference Abstract:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología.
doi: 10.3389/conf.fimmu.2015.05.00246
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Received:
30 May 2015;
Published Online:
14 Sep 2015.
*
Correspondence:
PhD. Renata Curciarello, CONICET-CCT La Plata- UNLP, Instituto de Inmunología y Fisiopatología, La Plata, Buenos Aires, Argentina, renata.curciarello@gmail.com