Role of acetylcholine on the isotype switching in Peyer's patches and lamina propria for IgA secretion in small intestine of BALB/c mice.
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1
Escuela Superior de Medicina, Instituto Politécnico Nacional, Sección de Investigación y Posgrado ESM-IPN, Mexico
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2
Escuela Superior de Medicina, Instituto Politécnico Nacional, Sección de Investigación y Posgrado ESM-IPN, Mexico
Background and aims. Gut-associated lymphoid tissue (GALT) is the most extensive and complex part of the immune system, wherein different neuroendocrine mechanisms are implicit in order to fulfill effectively with its barrier function. Acetylcholine (ACh), realised by vagus nerve, is a neuroimmunomodulator of intestinal mucosa that modifies the activity of immune cells according to the kind of cholinergic receptor that it stimulates. On one side, its binding to nicotinic receptors (nAChR) regulates proinflammatory cytokines production (cholinergic anti-inflammatory pathway); however, in vitro studies made in isolated hundles of small/large intestine showed that ACh also modulates secretory IgA (SIgA) release through muscarinic receptors (mAChR), which are located in lymphocytes T, lymphocytes B and epithelial cells. As the last response has been poorly studied in animal models, we made a truncal vagotomy in BALB/c mice in order to know the role of ACh on IgA secretion.
Methods. Twenty male mice underwent SHAM or truncal unilateral vagotomy. On 14th postoperative day, Peyer´s patches, lymphocytes of the lamina propria, intestinal liquid and plasma were harvested in order to determine total SIgA, lymphocytes B IgM+ (LB IgM+), lymphocytes B IgA+ (LB IgA+) and intracellular cytokines that allows IgA isotype switching (TGF-β, IL-4, IL-5, IL-6 e IL-10).
Results. Vagotomized mice had a decrease in sIgA levels, compared with SHAM and control group. Intracellular cytokines were also decreased after vagotomy. With SHAM surgery, sIgA levels were higher in proximal compared with distal small intestine; it was correlated with levels of intracellular cytokines (IL-4, -5, -10, TGF-β) and LB IgA+.
Conclusions. Vagus nerve has a greater influence on proximal segment and efector site. Unilateral troncular vagotomy removes an important source of ACh in small intestine. The decrease of SIgA, intracellular cytokines and LB IgA+ in proximal section, suggest that this neurotransmitter has an important role on synthesis and secretion of SIgA through muscarinic receptors activation.
Acknowledgements
This work was supported by SIP-COFAA IPN.
Keywords:
Neuroimmunomodulation,
Acetylcholine,
Mucarinic receptor,
Vagotomy,
IgA secretion
Conference:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.
Presentation Type:
Poster Presentation
Topic:
Mucosal Immunity and the microbiome
Citation:
Salas Pimentel
M,
Reséndiz Albor
AA,
Arciniega Martínez
IM,
Martínez Becerril
EM,
García Fonseca
A and
Campos Rodríguez
R
(2015). Role of acetylcholine on the isotype switching in Peyer's patches and lamina propria for IgA secretion in small intestine of BALB/c mice..
Front. Immunol.
Conference Abstract:
IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología.
doi: 10.3389/conf.fimmu.2015.05.00250
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Received:
16 May 2015;
Published Online:
14 Sep 2015.
*
Correspondence:
MD. Marisol Salas Pimentel, Escuela Superior de Medicina, Instituto Politécnico Nacional, Sección de Investigación y Posgrado ESM-IPN, Ciudad de México, Distrito Federal, 11340, Mexico, solsp2030@hotmail.com