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Obtention of tetanus antitoxin hyperimmune sera in horses

Miranda C. Antonio1, 2*, Sánchez A. Rolando3, Cobos V. Dailin4, Pérez M. Oliver5 and Góngora A. Walter6
  • 1 Centro de Inmunología y Biopreparados de Holguín. LABIOFAM, Research and Development, Cuba
  • 2 Universidad de Ciencias Médicas de Holguín, Research, Cuba
  • 3 Universidad de Ciencias Médicas de Holguín, Resaerch, Cuba
  • 4 Centro de Inmunología y Biopreparados de Holguín. LABIOFAM, Cuba
  • 5 Instituto de Ciencias Médicas Girón. Universidad de La Habana, Cuba
  • 6 Centro de Inmunología y Biopreparados, Cuba

Tetanus is an illness caused by the neurotoxin produced by the Clostridium tetani and its control is a strategy of the vaccination programs developed by the Panamerican Health Organization. For the production of tetanus toxoid are used international standards of antitoxins gotten in equines, however, these antisera are not available for their routine use in Cuba. The purpose of this study was to obtain a potent antitoxin against tetanus toxin. Four groups of mares were immunized. For the immunization, the different amounts of immunogen were emulsified in Immune- stimulating oil emulsion and injected according to the several multisite immunization protocols in low volumes. The second immunizations were carried out in Immune- stimulating oil emulsion. The antibody response was evaluated by Ramón flocculation assay. The antitoxin antibody titers varied significantly from an immunization protocol to another according to the studied parameters. Nevertheless, the same antitoxin titers increased quickly and reached a plateau around the 6 weeks for three groups of animals. The antibody titers were significantly higher in the fourth group of animals, reaching their activity to 2000 LfeqU/mL. We could conclude that the antitoxin antibody concentration increases with the time and the repetition of the immunogenic stimulus, and it is demonstrated that immunization protocols more lingering produce better outputs of antitoxins. This potent antitetanus toxin should be useful as internal reference material in the tetanus toxiod antigen production.

References

Chotwiwatthanakun C, R Pratanaphon, S Akesowan, S Sriprapat, K Ratanabanangkoona. 2001. Production of potent polyvalent antivenom against three elapid venoms using a low dose, low volume, multi-site immunization protocol. Toxicon 39: 1487-1494
Conclusiones de la Reunión de la Red Regional de Laboratorios Nacionales de Control de la Calidad de Vacunas. Caracas, Venezuela. 2005: Marzo 1-3:1-5.
Control de la difteria, la tos ferina, el tétanos, la infección por Hemophilus influenzae tipo B y la hepatitis B. Guía Práctica. Publicación Científico y Técnica 604. OPS. Oficina Regional OMS. 2006.
Cuba. Ministerio de Ciencia Tecnología y Medio Ambiente. Resolución 112 del Decreto Ley 190. Reglamento para el establecimiento de los requisitos y procedimientos de seguridad biológica en las instalaciones en las que se hace uso de animales y plantas con riesgo biológico. Capítulo 2 artículo 5. La Habana 2003.
Guidelines on: antibody production. CCAC. 2002. Consultado 24/11/2011, http://www.ccac.ca.
Lambrecht BN, M Kool, MAM Willart, H Hammad. 2009. Mechanism of action of clinically approved adjuvants. Current Opinion in Immunology 21, 23-29.
López Y, S Sifontes, JF Infante, D Díaz, M Obaya, E Sosa y cols. 2007. Evaluación de la toxicidad por dosis única de la vacuna antitetánica vax-TET en ratas Sprague Dawley. VacciMonitor 16 (3):19-23.
Miranda AR, R Sánchez, W Góngora, O Mulet. 2006. Obtención de sueros hiperinmunes de origen equino antitoxina tetánica, utilizando adyuvante oleoso. Correo Científico Médico de Holguín 10 (3). (http://www.cocmed.sld.cu/. Consultado 24/11/2011).
Shantavasinkul, P.; Tantawichien, T.; Jaijaroensup, W.; Lertjarutorn, S.; Banjongkasaena, A.; Wilde, H.; Sitprija, V. A 4-Site, Single-Visit Intradermal Postexposure Prophylaxis Regimen for Previously Vaccinated Patients: Experiences with 15000 Patients. Clinical Infectious Diseases, v. 51, n. 9, p. 1070–1072, 2010.
Thommen, I.; Sartor, M.; Moleta-Colodel, E.; Albuquerque, D. Adjuvant activity of rice oil on the immune response to ovalbumin. VacciMonitor, v. 20, n. 2, p. 1-5, 2011.
Wahby, A.; Abd El-Aziz, D.A.; Bashandy, A.H.; Ibrahim, N.M.; Mahdy, E.M. A low dose multi-site immunization schedule for raising therapeutic antivenom to the egyptian cobra naja haje venom. Egyptian Journal of Natural Toxins, v. 4, n. 2, p. 1-11, 2007.

Keywords: Tetanus Toxoid, Immune- stimulating oil emulsion, Equine antitoxin, antitetanus toxin, multisite immunization protocols

Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015.

Presentation Type: Poster Presentation

Topic: Veterinary and Comparative Immunology

Citation: Antonio MC, Rolando SA, Dailin CV, Oliver PM and Walter GA (2015). Obtention of tetanus antitoxin hyperimmune sera in horses. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00375

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Received: 13 May 2015; Published Online: 16 Sep 2015.

* Correspondence: PhD. Miranda C Antonio, Centro de Inmunología y Biopreparados de Holguín. LABIOFAM, Research and Development, Holguín, Cuba, antonio@cibho.hlg.sld.cu