Characterization of a new fish-derived bioactive neuropeptide involved in bone remodelling. Its physiological function and therapeutic potential.
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1
Institute of Marine Research, Aquatic Molecular Pathobiology, Spain
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2
University of Vigo, Biochemistry, Genetics and Inmunology, Spain
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3
California Institute of Technology, Division of Biology, United States
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4
Institute of Aquaculture Torre de la Sal, Fish Physiology and Biotechnology, Spain
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5
Centre of Marine Sciences, Comparative and Molecular Endocrinology, Portugal
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6
University of Maryland, Department of Molecular and Cellular Biology, United States
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7
University of Barcelona, Department of Genetics, Spain
A complex network of autocrine and paracrine signals, hormones and neuronal factors preserve the structural integrity of the skeleton and regulate mineral metabolism in vertebrates. We have characterized a new neuropeptide belonging to parathyroid hormone (PTH) family. PTH family members are known to play a key role in maintaining mineral homeostasis, bone remodeling and in regulating embryonic development of skeleton and other tissues. This new neuropeptide is synthesized by two clusters of neurons located in lateral hypothalamus as showed in whole mount in situ hybridization. The functional characterization of the gene using a stable transgenic line revealed its key role in the regulation of bone mineral density. Moreover, phylogenetic analyses and comparative genomics results of conserved synteny reveal that this new neuropeptide is a new ohnolog of the PTH family present in teleosts and some tetrapods like chicken, but absent in mammals . Our findings suggest a new brain to bone pathway, where neuronal factors from hypothalamus signal to receptors on bone cells promoting bone remodeling. Further investigations about this new neuropeptide system would be relevant for developing therapies for bone mineral disorders in humans, since this neuropeptide has a conserved domain similar to other PTH-related peptides which have anabolic effects on bone.
Acknowledgements
This work was funded by the Spanish Science and Innovation Ministry project AGL2008-00392/ACU and ALG2011-23581 to JR.
Keywords:
Bone remodelling,
mineral homeostasis,
Hypothalamus,
Neuropeptide,
Skeletal disorders,
Parathyroid hormone family
Conference:
IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014.
Presentation Type:
Oral Presentation
Topic:
BLUE BIOTECH
Citation:
Suarez-Bregua
P,
Torres-Nuñez
E,
Prober
D,
Cerdá-Reverter
J,
Guerreiro
PM,
Du
S,
Cañestro
C and
Rotllant
J
(2014). Characterization of a new fish-derived bioactive neuropeptide involved in bone remodelling. Its physiological function and therapeutic potential..
Front. Mar. Sci.
Conference Abstract:
IMMR | International Meeting on Marine Research 2014.
doi: 10.3389/conf.fmars.2014.02.00057
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Received:
25 Apr 2014;
Published Online:
18 Jul 2014.
*
Correspondence:
Miss. Paula Suarez-Bregua, Institute of Marine Research, Aquatic Molecular Pathobiology, Vigo, Pontevedra, 36208, Spain, paulasuarezbregua@gmail.com