High antimicrobial activity of VLC isolated fractions from Bifurcaria bifurcata
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1
School of Tourism and Maritime Technology, GIRM - Marine Resources Research Group, Polytechnic Institute of Leiria, Portugal
Infectious diseases continue to be a major public health problem and consequently there is perpetual need for new drugs. In this current study, the antimicrobial potential of isolated fractions from Bifurcaria bifurcata collected in Peniche coast was evaluated. Nine fractions of Bifurcaria bifurcata dichloromethane extracts were produced by Vacuum Liquid Chromatography (VLC) using cyclohexane with increasing amounts of 10% of ethyl acetate. All of the VLC fractions were tested against four microorganisms, namely Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 12600), Candida albicans (ATCC 10231) and Bacillus subtilis (ATCC 6633). Ampicillin, Chloramphenicol and Amphotericin B was used as positive control. In antimicrobial assays, the F4 fraction showed the best result with IC50 38.28 µg/ml (20.18-72.60) against B. subtilis growth. In same microorganism, the fractions F2 (IC50:183.1 µg/ml (110.5-305.6)), F3 (IC50: 71.56 µg/ml (48.10-106.4)) and F5 ((IC50: 95.23 µg/ml (60.02-151.1)) also inhibited the B. subtilis growth. The fractions F3 (IC50: 184.3 µg/ml (94.82-358.1)), F4 (IC50: 113.5 µg/ml (67.76-190.0)) and F5 (IC50: 439.6 µg/ml (249.4-774.8)) exhibited the highest activity against S. aureus. The fractions F3 and F4 inhibited the C. albicans growth with IC50 of 285.2 µg/ml (146.1-557.1) and 133.6 µg/ml (66.73-267.5), respectively. By contrast, all fractions need more than 1mg/ml to inhibit 50% of E. coli growth. In conclusion, the Bifurcaria bifurcata fractions, collected in Peniche, can be used as source of molecules with a great antimicrobial activity.
Keywords:
marine environment,
natural compounds,
Bifurcaria bifurcata,
Antibacterial activity,
macro-algae
Conference:
IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014.
Presentation Type:
Poster Presentation
Topic:
BLUE BIOTECH
Citation:
Horta
A,
Pandeirada
R,
Fernandes
L,
Alves
C,
Pinteus
S,
Silva
J and
Pedrosa
R
(2014). High antimicrobial activity of VLC isolated fractions from Bifurcaria bifurcata.
Front. Mar. Sci.
Conference Abstract:
IMMR | International Meeting on Marine Research 2014.
doi: 10.3389/conf.fmars.2014.02.00070
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Received:
10 May 2014;
Published Online:
18 Jul 2014.
*
Correspondence:
Dr. Andre Horta, School of Tourism and Maritime Technology, GIRM - Marine Resources Research Group, Polytechnic Institute of Leiria, Peniche, 2520–641, Portugal, andre.horta@ipleiria.pt