Molecular and biometric characterization of natural bream × roach hybrids population in the Dobczyce Reservoir (S Poland)
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1
Institute of Nature Conservation, Polish Academy of Sciences, Poland
Natural hybrids of leuciscine cyprinids were investigated in a medium-sized submontane reservoir (49°52'N, 20°03'E, altitude 270 m) in the Carpathian part of the Vistula basin. The material included 380 hybrid specimens (TL: 24.0–39.8 cm, SL: 19.0–31.7 cm, W: 134–714 g) collected in 2006–2013.
To detect their ancestry, genotypes of the 327 putative hybrids were compared to reference genotypes of 85 roaches, 115 common breams, 18 rudds, and 99 silver breams. Individuals were typed in 15 polymorphic microsatellite loci (Dubut et al. 2009), while species specific amplification of a fragment of mitochondrial cytochrome b gene was used to detect maternal origin of hybrids (Wyatt et al. 2006). Few individuals with traces of rudd and silver bream ancestry were excluded from the study.
Analysis of mitochondrial DNA suggests bias towards bream maternal origin, with only 22 individuals (6,7%) with roach mitochondrial DNA. The analyzed set of hybrids was composed mostly of first generation bream × roach hybrids. Only 7 individuals (2,1%) showed a sign of backcrossing to bream, however, this finding has to be confirmed by analysis of further loci as it might result from presence of null alleles in roach. F2 generation hybrids were not detected.
Biometric investigation of collected specimens comprised of selected morphometric (body height, and lengths of head, trunk, and tail) and meristic characters (counts of scales in lateral line and soft rays in anal fin) of individuals of different maternal origin. As all the analyzed differences between these categories of specimens appeared insignificant, the investigated population of hybrids may be regarded morphologically uniform.
References
Dubut, V., Martin, J.F., Gilles, A., Van Houdt, J., Chappaz, R., Costedoat, C. (2009). Isolation and characterization of polymorphic microsatellite loci for the dace complex: Leuciscus leuciscus (Teleostei: Cyprinidae). Mol. Ecol. Resour. 9(4): 1179-1183. doi: 10.1111/j.1755-0998.2009.02594.x.
Wyatt, P.M.W. , Pitts, C.S., Butlin, R.K. (2007). A molecular approach to detect hybridization between bream Abramis brama, roach Rutlius rutilus and rudd Scardinius erythrophthalmus. J. Fish Biol. 69(sa):52 - 71. doi: 10.1111/j.1095-8649.2006.01104.x.
Keywords:
Dam reservoirs,
Hybridization, Genetic,
Roach,
Common bream,
morphometry,
microsatellites
Conference:
XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015.
Presentation Type:
Poster Presentation
Topic:
Phylogeny, Systematics and Genetics
Citation:
Konopiński
MK and
Amirowicz
A
(2015). Molecular and biometric characterization of natural bream × roach hybrids population in the Dobczyce Reservoir (S Poland).
Front. Mar. Sci.
Conference Abstract:
XV European Congress of Ichthyology.
doi: 10.3389/conf.fmars.2015.03.00112
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Received:
20 Nov 2015;
Published Online:
22 Nov 2015.
*
Correspondence:
Dr. Maciej K Konopiński, Institute of Nature Conservation, Polish Academy of Sciences, Kraków, 31-120, Poland, konopinski@iop.krakow.pl