Ontogenetic stomach development in catshark Scyliorhinus canicula.
Odete
Goncalves1, 2*,
Filipe
Castro1*,
Renata
Freitas3,
Patricia
Ferreira1, 2,
Ana
Aguas2, 3,
Fabio
Barroso2, 3,
Sylvie
Mazan4,
João
Coimbra1, 2 and
Jonathan
M.
Wilson1, 5*
-
1
CIIMAR, Portugal
-
2
ICBAS, Portugal
-
3
IBMC, Portugal
-
4
Station Biologique de Roscoff, France
-
5
Wilfrid Laurier University, Canada
The gastrointestinal tract (GIT) of vertebrates develops from a simple undifferentiated tube into highly differentiated regions for the digestion and absorption of nutrients. Each region has a different histological structure and gene expression profile that enables the performance of their different functions. The stomach is the most highly derived GIT region and gastric glands are responsible for the secretion of HCl and pepsinogen that enables acid-peptic digestion, representing a functional innovation found exclusively in jawed vertebrates. The mechanisms of GIT organ specification are well studied in some vertebrates including mouse, Xenopus, chicken and some teleost fishes. General embryonic development has been studied in chondrichthyans but not GIT development. In this study we characterize the development of the stomach in the catshark Scyliorhinus canicula, an elasmobranch with a ~5 month development spanning 34 developmental stages. As a representative of basal jawed vertebrate for which genomic information is currently available, the catshark is a suitable model system to characterize the ancient expression and function developmental of physiologically relevant genes. Therefore, during this study we used embryos from a brood stock held in our fish facility to conduct gene expression, protein detection and histological analyses. Our aim was to characterize, at molecular and morphological levels, stomach development and the differentiation of its characteristic secreting glands. The differentiation of the stomach starts around stage 24. However, immunohistochemistry for the gastric proton pump indicates a delayed development of gastric gland that are only visible before hatching at stage 33-34. Supported by NSERC to JMW
Acknowledgements
BOGA-CIIMAR
FCT (grant BD/79821/2011)
NSERC (Canada)
References
Ballard WW, Mellinger J, Lechenault H (1993) A series of normal stages for development of Scyliorhinus canicula, the lesser spotted dogfish (Chondrichthyes; Scyliorhinidae). J Exp Zool. 267:318–3362
Freitas, R, Zhang GJ and Cohn, MJ (2006). Evidence that mechanisms of fin development evolved in the midline of early vertebrates. Nature 442, 1033-1037.
Castro L.F.C., Goncalves O, Mazan S, Tay B-H, Venkatesh B, Wilson JM (2014). Recurrent gene loss correlates with the evolution of stomach phenotypes in gnathostome history. Proceedings of the Royal Society B-Biological Sciences 281: 1775.
Keywords:
Stomach,
Development Studies,
vertebrate,
Chondrichthyes,
Immunohistochemistry
Conference:
XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015.
Presentation Type:
Poster Presentation
Topic:
Physiology, Behavior and Toxicology
Citation:
Goncalves
O,
Castro
F,
Freitas
R,
Ferreira
P,
Aguas
A,
Barroso
F,
Mazan
S,
Coimbra
J and
Wilson
JM
(2015). Ontogenetic stomach development in catshark Scyliorhinus canicula..
Front. Mar. Sci.
Conference Abstract:
XV European Congress of Ichthyology.
doi: 10.3389/conf.FMARS.2015.03.00261
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Received:
31 Dec 2015;
Published Online:
31 Dec 2015.
*
Correspondence:
MD. Odete Goncalves, CIIMAR, Porto, Portugal, odete007@gmail.com
PhD. Filipe Castro, CIIMAR, Porto, Portugal, lfilipecastro@gmail.com
Dr. Jonathan M Wilson, CIIMAR, Porto, Portugal, wilson.jm.cimar@gmail.com