Tiahuramides, novel compounds isolated from Lyngbya majuscula, display cytotoxic effects in human neuroblastoma cells
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1
Universidad de Santiago de Compostela, Departamento de Farmacología, Facultad de Veterinaria, Spain
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2
Université de Perpignan, CRIOBE, USR CNRS-EPHE-UPVD 3278, France
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3
Ocean Nutrition Canada, Dartmouth, Nova Scotia, B2Y 4T6, Canada
Three new compounds, named tiahuramides A, B and C, were isolated from the marine cyanobacteria Lyngbya majuscula. This filamentous eubacteria has been a source of several secondary metabolites with different biological activities. Lyngbya majuscula compounds have shown cytotoxicity against cancer cell lines, anti-inflammatory activity, blockage of the voltage gated sodium channels or induction of actin polymerization. In this study, we tested the cytotoxicity of the novel compounds tiahuramides A, B and C in human neuroblastoma SH-SY5Y cells. Neuroblastoma cells were incubated with compounds for 24 h with concentrations ranging from 100 µM to 1 nM and the half maximal inhibitory concentration (IC50) was determined. Tiahuramides B and C showed an IC50 of 13.8 and 6.0 µM, respectively, whereas tiahuramide A showed no toxicity in this cell line. In order to better understand the type of cell death induced by compounds, neuroblastoma cells were coincubated with tiahuramides B, C and the caspase inhibitor zVAD. Cell viability showed no significant differences between cells cotreated with tiahuramides and zVAD and cells treated with compounds alone, indicating that the cytotoxicity produced by tiahuramides is not related with apoptotic cell death. In view of these results, SH-SY5Y cells treated with compounds were stained with Annexin V-FITC and propidium iodide and analysed by flow cytometry. The results obtained with this double staining confirmed that tiahuramides B and C produce cell death in human neuroblastoma cells through necrosis.
Acknowledgements
This research has received funding from the following FEDER cofunded-grants. From CDTI and Technological Funds, supported by Ministerio de Economía y Competitividad, AGL2012-40185-CO2-01, AGL2014-58210-R, and Consellería de Cultura, Educación e Ordenación Universitaria, GRC2013-016. From CDTI under ISIP Programme, Spain, IDI-20130304 APTAFOOD. From the European Union’s Seventh Framework Programme managed by REA – Research Executive Agency (FP7/2007-2013) under grant agreement 312184 PHARMASEA
Keywords:
Lyngbya majuscula,
tiahuramides,
Cytotoxicity,
SH-SY5Y,
Necrosis
Conference:
IMMR | International Meeting on Marine Research 2016, Peniche, Portugal, 14 Jul - 15 Jul, 2016.
Presentation Type:
Poster presentation
Topic:
Blue Biotechnology
Citation:
Alvariño
R,
Alonso
E,
Abou Mansour
E,
Bornancin
L,
Bonnard
I,
Banaigs
B,
Alfonso
A and
Botana
LM
(2016). Tiahuramides, novel compounds isolated from Lyngbya majuscula, display cytotoxic effects in human neuroblastoma cells.
Front. Mar. Sci.
Conference Abstract:
IMMR | International Meeting on Marine Research 2016.
doi: 10.3389/conf.FMARS.2016.04.00064
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Received:
13 Jul 2016;
Published Online:
13 Jul 2016.
*
Correspondence:
Dr. Luis M Botana, Universidad de Santiago de Compostela, Departamento de Farmacología, Facultad de Veterinaria, Lugo, Lugo, 27002, Spain, luis.botana@usc.es