Fucus spiralis growth in artificial conditions revealed high antioxidant potential.
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1
MARE – Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, Portugal
Fucus spiralis is a brown alga (Heterokontophyta, Phaeophyceae), living on the littoral shore of the Atlantic coasts of Europe and North America, and is commonly found in the Portuguese coast. It has the common names of spiral wrack and flat wrack [1]. Previous works have revealed the enormous potential of Fucus spiralis as regards to its antioxidant capacity [2]. Since the synthesis of antioxidant compounds continues to be very challenging and the collection from the marine environment is unsustainable, one possible strategy to obtain the desired bioactive compounds may encompass the sustainable growth in artificial conditions.
In this work, Fucus spiralis was collected on Marques Neves beach (39°22'14.7"N 9°23'18.6"W), Peniche – Portugal, cleaned in place with seawater and immediately transported to laboratory. Visible healthy plants were cleaned with a manual brushing for removing any possible microscopic epiphytes and selected for tissue culture.
After the disinfection process, algae were thoroughly rinsed with autoclaved seawater and then excised into 4–5 mm length explants. Six visible healthy fragments were distributed per eight (n=8) 250 mL balloons with 100 mL of PES medium in constant sterile aeration). The cultures were maintained at two light intensities: 60-80 μmol m-2s-1 and 120-140 μmol m-2s-1 at 22 °C in a 12:12h light:dark photoperiod. To ensure sustainable growth, the explants were transferred regularly to fresh medium at 15-days intervals during 120 days.
The antioxidant potential of the grown callus was assessed by quantifying the total phlorotannins production by the Folin-Ciocalteu method. The results were compared with wild type.
The wild type Fucus spiralis presented the highest phlorotannins production with ≈350 Phloroglucinol equivalents (PE) per gram of extract. However, the callus obtained in artificial conditions produced ≈ 250 and 150 PE per gram of extract, in light intensity of 60-80 μmol m-2s-1 and 120-140 μmol m-2s-1, respectively.
The present study provides useful data in clonal propagation of Fucus spiralis cultivation. These results open a new window of possibilities for algae culturing in order obtain bioactive compounds in a sustainable and environmental friendly way.
Acknowledgements
This work was funded by FP7 EU project “BAMMBO: Sustainable production of Biologically Active Molecules of Marine Based Origin” (FP7 nº 265896/ http//www.bammbo.eu). Celso Alves, Susete Pinteus and Joana Silva are financial supported by a grant from FCT - Fundação para a Ciência e Tecnologia (SFRH/BD/97764/2013, SFRH/BD/96203/2013, SFRH/BD/103255/2014, respectively).
References
1. www.algaebase.org
2. Pinteus, S., et al. "High antioxidant potential of Fucus spiralis extracts collected from Peniche coast (Portugal)." New Biotechnology 25 (2009): S296.
Keywords:
Phlorotannins,
Marine Natural Products,
Clonal propagation,
bioactive compounds,
seaweeds
Conference:
IMMR | International Meeting on Marine Research 2016, Peniche, Portugal, 14 Jul - 15 Jul, 2016.
Presentation Type:
Poster presentation
Topic:
Blue Biotechnology
Citation:
Pinteus
S,
Alves
C,
Horta
A,
Silva
J and
Pedrosa
R
(2016). Fucus spiralis growth in artificial conditions revealed high antioxidant potential..
Front. Mar. Sci.
Conference Abstract:
IMMR | International Meeting on Marine Research 2016.
doi: 10.3389/conf.FMARS.2016.04.00066
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Received:
14 May 2016;
Published Online:
13 Jul 2016.
*
Correspondence:
Dr. Susete Pinteus, MARE – Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, Peniche, 2520-641, Portugal, susete.pinteus@ipleiria.pt