Effect of temperature and nitrogen concentration on the heterotrophic growth of Chlorella vulgaris
-
1
CMP - Algafarm, Microalgae Production Plant, Algafarm, Portugal
-
2
ESTM, Instituto Politécnico de Leiria, MARE – Marine and Environmental Sciences Centre, Portugal
-
3
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado, Portugal
-
4
Necton, Companhia Portuguesa de Culturas Marinhas, S.A., Portugal
Worldwide, microalgae are cultivated in autotrophic growth conditions, since all algae are photosynthetic and efficient light converters. Nevertheless, heterotrophic growth has been gaining importance in all the stages of the scale up: from lab to industrial scale conditions. Several advantages have been demonstrated. Growth under heterotrophic conditions effectively controls the contamination issue, enhances the productivity (higher growth rates), increases the yield of several secondary metabolites resulting in an overall biomass cost reduction. Despite of that, not all microalgae are capable to grow heterotrophically.
CMP owns Algafarm located in Pataias, Leiria. Algafarm is the biggest microalgae production facilities in Europe working with closed systems. Presently Algafarm is focused in the capture of CO2 by microalgae. Higher productivities mean higher capture rates. Heterotrophic production of the photobioreactor inocula is one of the variables that is under study. In this context, the present work aimed at finding: i) the optimum temperature for heterotrophic growth of C. vulgaris UTEX 2714 and ii) the optimum N-source for heterotrophic growth of the same strain.
Chlorella vulgaris was cultivated heterotrophically at 21, 26 and 28 ºC with two different N-source media. Results showed that independently of the growth medium. Under the tested conditions the best temperature to grow this microalgae was 28ºC. Lipid content significantly increased when cultures grow only with ammonia as N-source. Chlorophyll content varies with growth medium but no differences between the three tested temperatures were observed.
Keywords:
Chlorella vulgaris,
heterotrophy,
Lipids,
Chlorophyll,
Growth medium
Conference:
IMMR | International Meeting on Marine Research 2016, Peniche, Portugal, 14 Jul - 15 Jul, 2016.
Presentation Type:
Oral presentation
Topic:
Blue Biotechnology
Citation:
Marques
AE,
Barros
A,
Correia
CR,
Silva
J,
Navalho
J,
Leandro
SM and
Gomes
R
(2016). Effect of temperature and nitrogen concentration on the heterotrophic growth of Chlorella vulgaris.
Front. Mar. Sci.
Conference Abstract:
IMMR | International Meeting on Marine Research 2016.
doi: 10.3389/conf.FMARS.2016.04.00121
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
29 Apr 2016;
Published Online:
13 Jul 2016.
*
Correspondence:
Dr. Ana E Marques, CMP - Algafarm, Microalgae Production Plant, Algafarm, Pataias, 2445 - 411, Portugal, ana.marques@allmicroalgae.com