Genetic and morphometric variability of the invasive shrimp Macrobrachium rosemberguii in Maranhão State Coast – Brazil
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1
Universidade Estadual do Maranhão, Curso de Pós Graduação em Recursos Aquáticos e Pesca, Brazil
Macrobrachium rosemberguii is a shrimp from Indo-Pacific region cultivated in many countries including Brazil. The species is considered an exotic invader due to escapes that allowed the species to invade many natural environments of the Brazilian coast. In Maranhão (Northern Coast of Brazil) the Giant Malaysian Prawn can now be found throughout the coast to the low lands, occupying areas of extreme ecological importance. This study aimed to investigate the genetic and morphometric variability of M. rosemberguii introduced in the coast of Maranhão. We examined 110 individuals collected in five points of the estuarine area: Primeira Cruz, Humberto de Campos, Barreirinhas, Santo Amaro, São Bento. To the morphometric characterization we obtained the measures of the body dimensions using a caliper rule and calculated average sizes. A medium size of 232 mm was recorded for the females and 137 mm, 209 mm and 238 mm for each male morphotype (small male, orange claw, blue claw, respectively). These results indicate bigger animals than registered by Piedade (2008) in Maranhão area. To do the genetic characterization, we obtained fragments of COI gene from 46 animals. Genomic DNA was extracted from samples using a standard saline protocol; fragments were amplified by the PCR using primers described by Folmer et al (1994) and sequenced using ABI-PRISM 3100 automated sequencer. Alignments were checked and edited by hand. Comparisons and measures of variability were performed using Mega. A 545 bp sequence of the COI region was obtained, defining 3 different haplotypes. Low levels of nucleotide diversity (Pi = 0,005) were observed among individuals. Haplotype diversity was 0,047 and divergence between populations was 0,005 - 0,009. Two of the haplotypes were in agreement with the described by Iketani (2010) from Macrobrachium introduced in Brazil. The third haplotype, on the other hand, represent a new haplotype from Brazilian coast. The hereby data contribute to understand the process of biological invasion which are determinant events of health and composition of aquatic communities.
Acknowledgements
Fundação de Amparo ao Desenvolvimento Científico e Tecnológico do Maranhão - FAPEMA
References
IKETANI, G.; PIMENTE, L.; SILVA-OLIVEIRA, G.; MACIEL, C.; VALENTI, W.; SHNEIDER, H.; SAMPAIO, I (2011) The introduction of the gigante river prawn, Macrobrachium rosenbergii (Decapoda, Palaemonidae) in Brazil: New insights from molecular data. Genetics and Molecular Biology, v. 34, n. 1, p. 142-151.
PIEDADE, RNJ (2008) Avaliação Sócio-Ambiental da Introdução do Camarão Exótico Macrobrachium rosenbergii (De Man, 1879) (Decapodo, Palaemonidae) em Águas Naturais da Região Lacustre de Penalva, APA da Baixada Maranhense. Monografia de Conclusão de Curso, Ciências Aquáticas. Universidade Federal do Maranhão.
Keywords:
biological invasions,
Crustacea,
Genetic Variation,
morphometric,
Brazilian coast
Conference:
XIX Iberian Symposium on Marine Biology Studies, Porto, Portugal, 5 Sep - 9 Sep, 2016.
Presentation Type:
Poster Presentation
Topic:
1. ECOLOGY, BIODIVERSITY AND VULNERABLE ECOSYSTEMS
Citation:
Tchaicka
L,
Alves
JJ,
Fonseca
LA,
Paz
F,
França
I,
Almeida
ZD,
Carvalho
RF and
Araujo
A
(2016). Genetic and morphometric variability of the invasive shrimp Macrobrachium rosemberguii in Maranhão State Coast – Brazil.
Front. Mar. Sci.
Conference Abstract:
XIX Iberian Symposium on Marine Biology Studies.
doi: 10.3389/conf.FMARS.2016.05.00144
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Received:
30 Apr 2016;
Published Online:
03 Sep 2016.
*
Correspondence:
Dr. Ligia Tchaicka, Universidade Estadual do Maranhão, Curso de Pós Graduação em Recursos Aquáticos e Pesca, São Luis, Maranhão, Brazil, ltchaicka@yahoo.com.br