Biotechnological potential of Sulfated Polysaccharides isolated from macroalgae of the Peniche coast
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1
MARE – Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, Portugal
The marine biome is home to several ecosystems with a vast amount of organisms which have already shown to produce bioactive compounds. Within, macroalgae have been studied revealing to be an interesting source of new compounds with biological activities. Among these compounds, sulfated polysaccharides have demonstrated to possess interesting bioactivities with potential to be used in the development of new therapeutic approaches. Thus the main aim of this study was to evaluate the antioxidant and antimicrobial activities of the sulfated polysaccharides (SP) isolated from two brown macroalgae, Sargassum muticum and Bifurcaria bifurcata, collected on the Peniche coast (Portugal). The SP were extracted from the macroalgae by an enzymatic process [1]. The antioxidant activity was evaluated through three methods: Ferrous reducing capacity / antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and oxygen radical absorption capacity (ORAC). The antimicrobial activity was evaluated through the determination of inhibitory concentration (IC50) in liquid growth medium by spectrophotometric reading against Staphylococcus aureus and Pseudomonas aeruginosa. The antioxidant activity of Sargassum muticum SP showed the highest FRAP with 107.51 ± 0.15 μM FeSO4 equivalents per gram of extract, and an IC50 of 34.47 ± 7.07 μg mL-1 and 349.29 ± 5.24 μmol of Trolox equivalents per gram of extract through DPPH and ORAC methods, respectively. Regarding the antibacterial activity, the results were more expressive against S. aureus with IC50 values of 17.02 (10.04-28.86) µg mL-1 and 45.32 (34.31-59.85) µg mL-1, for B. bifurcata and S. muticum SP, respectively. S. muticum SP also inhibited the growth of P. aeruginosa showing an IC50 of 818.10 (669.50-999.50) µg mL-1. In conclusion the SP of Sargassum muticum revealed the greater antioxidant activity and antimicrobial activity against S. aureus (Gram+) and the SP of Bifurcaria bifurcata demonstrated the highest activity against P. aeruginosa (Gram-).
Acknowledgements
Authors would like to acknowledge the financial support of the Portuguese Foundation for Science and Technology (FCT) through Strategic Project UID/MAR/04292/2013 granted to MARE – Marine and Environmental Sciences Centre, through Red2Discovery Project (PTDC/MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145-FEDER-016791), and through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015 - LISBOA-01-0145-FEDER-016405).
References
1-Souza, R. B., Frota, A. F., Silva, J., Alves, C., Neugebauer, A. Z., Pinteus, S., Rodrigues, J. A. G., Cordeiro, E. M. S., Almeida, R., Pedrosa, R. & Benevides, N. M. B. (2018). In vitro activities of kappa-carrageenan isolated from red marine alga Hypnea musciformis: Antimicrobial, anticancer and neuroprotective potential. International journal of biological macromolecules, 112, 1248-1256.
Keywords:
macroalgae,
Sargassum muticum,
Bifurcaria bifurcata,
Antibacterial activity,
antioxidant activity,
sulfated polysaccharides
Conference:
IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018.
Presentation Type:
Poster Presentation
Topic:
Blue Biotech
Citation:
Gomes
F,
Teodoro
F,
Silva
J,
Pinteus
S,
Alves
C,
Bernardino
SM and
Pedrosa
RP
(2019). Biotechnological potential of Sulfated Polysaccharides isolated from macroalgae of the Peniche coast.
Front. Mar. Sci.
Conference Abstract:
IMMR'18 | International Meeting on Marine Research 2018.
doi: 10.3389/conf.fmars.2018.06.00164
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Received:
27 Jul 2018;
Published Online:
07 Jan 2019.
*
Correspondence:
PhD. Rui P Pedrosa, MARE – Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, Peniche, Portugal, rpedrosa@ipleiria.pt