Imaging RNA in living neural circuits with hybridization-sensitive fluorescent probes
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1
Kyoto University, Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Japan
Photoquenching-based fluorescence imaging is becoming increasingly important in live-cell imaging. Highly sensitive cameras in combination with powerful computational methods have made possible detections of a single target molecule in a highly noisy cellular living environment. The improvement has allowed, for the first time, effective detection of RNA molecules that can be expressed at a single copy or at thousands of copies at a given time in a living cell. Taking advantage of the technical improvement, we aim to detect activity-triggered changes in gene expression in the brain using photoquenching techniques in order to dissect the molecular and cellular basis of learning. If we can “see” newly synthesized RNA in a living brain, we will have a sense of real-time interactions between environment and genome. If we can resolve gene expression at single-cell level, we will know what neural circuits are integrating synaptic inputs to genome and are making long-lasting changes to their connectivity with newly expressed genetic information. In my presentation, I will introduce two newly developed RNA labeling technologies that may be potentially used to achieve such goals in living animal brains.
Keywords:
RNA,
imaging,
neural circuits,
single cell analysis,
photoquenching-based fluorescence imaging
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Symposium 4: The Living Chemistry of RNA
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Wang
DO
(2016). Imaging RNA in living neural circuits with hybridization-sensitive fluorescent probes.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00017
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Received:
26 Jul 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Dan O Wang, Kyoto University, Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Sakyo-ku, Kyoto, Japan, dwang@icems.kyoto-u.ac.jp