The effects of fluoxetine on long term potentiation in the hippocampal dentate gyrus granule cells male rats
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1
Hamadan university of Medical Sciences, Neurophysiology Research Center, Iran
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2
Hamadan University of Medical Sciences, Students Research Center, Iran
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3
Hamadan university of Medical Sciences, Faculty of Medicine , Iran
There are paradoxical reports about treatment with selective serotonin re-uptake inhibitors (SSRIs) on different types of learning and memory. Hippocampus-dependent memory is mediated by hippocampal synaptic plasticity, such as long-term potentiation (LTP). In present study, we examined the effects of acute administration of fluoxetine, as one of the popular SSRIs, on the evoked potentials and LTP induction in the hippocampal dentate gyrus of rats. Thirty male rats were randomly divided into sham saline treated, fluoxetine (2 mg/kg IP) and fluoxetine (10mg/kg IP) groups. The animals were anaesthetized and placed in a stereotaxic device for surgery and recording. The recording and stimulating electrodes were positioned in the granular cells of dentate gyrus (DG) and perforant pathway, respectively. After ensuring a steady state baseline response, single IP injection of treatment was done. The population spike (PS) amplitude and the slope excitatory postsynaptic potentials (EPSP) were compared between these three groups. In addition, paired pulses stimuli were applied to determine recurrent inhibition as a role of inhibitory interneuron. The results of basal activity showed that there was no difference in the EPSP and PS between three groups. However, there was a significant difference in the EPSP and PS between three groups following the tetanic stimulation. Augmentation of PS in the fluoxetine 2 and 10mg/kg was more than saline treated group. Potentiation in the EPSP of fluoxetine 2mg/kg, but not 10mg/kg was significantly less than saline group. Fluoxetine had no significant effects on the recurrent inhibition. These results indicate that acute administration of higher doses of fluoxetine (10mg/kg) can induce LTP. Thus, fluoxetine may be considered as a memory enhancer in the normal rats. This effect is not due to the disinhibition of inhibitory interneurons.
Keywords:
Fluoxetine,
Long-Term Potentiation,
Perforant Pathway,
plasticity,
population spike
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
YIC02: Young Investigator Colloquium 2
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Sadeghian
R,
Shahidi
S,
Rabiee
L and
Komaki
A
(2016). The effects of fluoxetine on long term potentiation in the hippocampal dentate gyrus granule cells male rats.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00060
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
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Correspondence:
Dr. Reihaneh Sadeghian, Hamadan university of Medical Sciences, Neurophysiology Research Center, Hamadan, Iran, re.sadeghian1414@gmail.com