The role of α-tocopherol transfer protein-tocopherol transfer protein (α-TTP) for the uptake of vitamin E in the prevention of glutamate induced injury in neuronal cells
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1
Universiti Putra Malaysia, Department of Biomedical Science, Faculty of Medicine and Health Sciences,, Malaysia
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2
Melaka Manipal Medical College , Foundation of Science, Malaysia
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3
Universiti Putra Malaysia, Deparment of Nutrition and Dietetic, Faculty of Medicine and Health Sciences, Malaysia
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4
Universiti Putra Malaysia, Department of Pathology, Faculty of Medicine and Health Sciences, , Malaysia
Vitamin E composed of eight different isomers of a, ß, γ, δ -tocopherols and tocotrienols. Human α-TTP is a cytosolic liver protein that plays a central role in vitamin E homeostasis with strong affinity to α-tocopherol. Despite of discriminated by α-TTP, α-tocotrienol provides the most potent neuroprotection amongst vitamin E. Tocotrienols has been shown to possess powerful antioxidant properties that can reduce effects of oxidative stress at nanomolar concentration. In view of the fact that the source of vitamin E for Malaysian is primarily from tocotrienol, therefore understanding of its uptake is rather crucial to ensure its benefit to the consumer. The aim of this study is to elucidate the behaviour of α-TTP in the condition with the absence of tocopherol, whether α-TTP will seek for tocotrienol. We assume that α-TTP is capable to take up tocotrienol in the condition with less availability of tocopherol. Cells were then treated with different ratio of Tocopherol (T) : Tocotrienol Rich fraction (TRF) (%): (100 T:0 TRF), (75 T:25 TRF), (50 T:50 TRF), (25T:75TRF), and (0T:100 TRF) and incubated for 12 and 24 hours respectively. RNA extraction was performed followed by reverse transcribe to cDNA. The expression of α-TTP upon treatment with different ratio of T:TRF was determined by Real Time Polymerase Chain Reaction (PCR) which amplify the DNA segment using α-TTP primer and GAPDH primer as a housekeeping gene and SYBR Green as a probe. The result was analyzed with statistical analysis one way ANOVA and post hoc Tukey test and p = 0.05 is considered statistical significant. α-TTP is capable to take up tocotrienol in the condition with less availability of tocopherol and we can postulate that α-TTP is not only responsible for tocopherol uptake but it is also involved in the uptake of other vitamin E isomers.
Keywords:
Gene Expression,
Vitamin E,
Neuron,
injury,
α-Tocopherol transfer protein
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Khaza'Ai
H,
Selvaraju
TR,
Abd Mutalib
M and
Vidyadaran
S
(2016). The role of α-tocopherol transfer protein-tocopherol transfer protein (α-TTP) for the uptake of vitamin E in the prevention of glutamate induced injury in neuronal cells.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00122
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Huzwah Khaza'Ai, Universiti Putra Malaysia, Department of Biomedical Science, Faculty of Medicine and Health Sciences,, Serdang, Selangor, Malaysia, huzwah@upm.edu.my