Role of phosphorylated delta sleep inducing peptide (p-DSIP) on sleep architecture and spatial memory impairment of rats under chronic hypobaric hypoxia
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1
Defence Institute of Physiology and Allied Sciences (DIPAS), Defence Research and Development Organization (DRDO), Neurophysiology, India
Sleep architecture alteration is one of the causes for sleep deprivation at high altitude. Hypobaric hypoxia (HH) affects non rapid eye movement sleep (NREM) followed by alteration in rapid eye movement sleep. NREM plays major role in spatial memory. Delta sleep inducing peptide (DSIP) is a well known peptide playing a role in improving sleep quality. Previous studies have shown HH induced memory impairments but its correlation with sleep deprivation is still need to be understood. The present study was designed to evaluate the role of sleep in HH induced cognitive declined. Animals were exposed to HH stress at 25,000 feet for continuous 7 days. P-DSIP was given as intervention at a dose of 10µg/kg/day for positive control. Norepinephrine (NE), serotonin and dopamine (DA) levels were measured from hippocampus, cortex, brain stem and hypothalamus by HPLC. Result showed that concentration of NE and DA were more increased after chronic hypoxia than control group. The finding may be due to over activity of tyrosine hydroxylase. Serotonin level was decreased in hypoxia group. It may be casued by the less activity of tryptophan hydroxylase as it is more sensitive to oxygen than monoamine oxidase (MAO). On the other hand, in p-DSIP treated group NE and DA levels significantly decrease than without drug treated group and serotonin level was significantly increases. Morris water maze (MWM) performance was significantly improved in p-DSIP treated hypoxic rats than only hypoxia group. MWM performance improvement may be due to improvement of serotonin level in drug treated group. P-DSIP has a major possibility to improve quality of sleep in drug treated group, which may directly play a positive role in MWM performance.
Keywords:
Memory,
Sleep,
neurotransmitters,
in vivo,
hypobaric hypoxia
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Roy
K,
Kishore
K,
Ray
K,
Wadhwa
M,
Jain
V,
Chauhan
G,
Kumari
P,
Alam
S,
Panjwani
U and
Singh
S
(2016). Role of phosphorylated delta sleep inducing peptide (p-DSIP) on sleep architecture and spatial memory impairment of rats under chronic hypobaric hypoxia.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00138
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Krishna Kishore, Defence Institute of Physiology and Allied Sciences (DIPAS), Defence Research and Development Organization (DRDO), Neurophysiology, Delhi, Delhi, India, kishu16in@yahoo.com