Morphology of human cerebellar cortex from anatomy cadaver preparations
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1
Universitas Airlangga, Faculty of Medicine, Indonesia
In our institution, the morphology of human cerebellum has yet determined well, however fresh samples are hard to obtain both for research and education purposes. Here we study the possibility to produce representative cerebellum histology sections out of anatomy cadavers that had been embalmed in 37% formalin fixative solution for over a year (n=3), in contrast to the usual 4-10% formalin solution used in routine histology staining. Arguably, prolonged and high concentration of formalin can alter the tissue composition and staining quality. Ethical clearance is obtained from the Ethic Committee of Faculty of Medicine, Universitas Airlangga. Cadavers (adult male, similar age and race, passed away due to natural causes without blatant brain injury) were embalmed with 37% formalin fixative solution for over a year before brain harvesting. After brain harvesting, cerebellum was removed, the cortex was sliced into cubicle of 1 cm of diameter and temporary soaked in 50-60% of alcohol and rinsed with running water before parrafinated. Two parasagittal adjacent slices (5µm) from each sample were deparaffinated and then stained with hematoxylin-eosin (HE). Slides were observed under the light microscope (Olympus, Japan). Pictures were taken from 6 field numbers and analysed with Optilab and Image Raster 3 softwares (Indonesia) at magnification of 100x and 400x. We observe a clear lamination of all 3 layers from all cerebellar cortices; molecular layer, piriform layer and the granular layer. The Purkinje cells and the granule cells can be seen clearly, both nuclei and the cytoplasm. The cytoskeletons of these cortices are nicely demonstrated, including the dendrites of the Purkinje cells and the neuropils of all layers. Limited artifacts were found, including rifts and blemish, although were still within acceptable range. Thus, it is possible to produce representative cerebellum histology slides from anatomy cadaver preparations in our institution.
Keywords:
Cerebellum,
Histology,
morphology,
Cadaver,
formalin
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Kalanjati
VP,
Dewi
AK and
Santoso
MA
(2016). Morphology of human cerebellar cortex from anatomy cadaver preparations.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00152
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Viskasari P Kalanjati, Universitas Airlangga, Faculty of Medicine, Surabaya, East Java, Indonesia, viskasari-p-k@fk.unair.ac.id