Regional extravasation of Evans blue dye in the brain across the blood-brain barrier during cerebral hypoperfusion
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1
Universiti Sains Malaysia, Centre for Drug Research, Malaysia
Cerebral hypoperfusion arises as a result of reduced net blood flow to the brain, disrupting the steady constant supply of oxygen and nutrients to the brain. As a result, a cascade of pathological changes occur in the brain that may then lead to the development of various neurological disorders including vascular dementia and Alzheimer’s disease. Reduced blood flow to the brain is often observed among the aging population and clinical patients with cardiovascular diseases and hypertension. The blood-brain barrier (BBB), which forms a dynamic physical, transport and enzymatic barrier between the brain parenchyma and circulating blood, is disrupted during cerebral hypoperfusion causing leakages of blood-borne substances into the brain parenchyma. To understand the vulnerability of different brain regions to BBB leakages during hypoxia insult, the regional extravasation of Evans blue dye (EBD) as an exogenous tracer was assessed in a rat model of bilateral carotid artery stenosis (BCAS) that mimics the condition of cerebral hypoperfusion in animal model. BCAS was induced in rats by narrowing both the common carotid arteries using microcoils then extravasation of EBD was measured by fluorimetry in brain regions of frontal cortex, striatum, posterior cortex, hippocampus, thalamus-midbrain and cerebellum. In the BCAS group, we observed marked extravasation of EBD in the brain regions of frontal cortex, posterior cortex, thalamus-midbrain and cerebellum in comparison to that of the sham group. These observations suggest the vulnerability of these regions towards hypoxia insult, further confirming the role of BBB disruption as an initiating aetiology for the development of more severe neurovascular diseases following cerebral hypoperfusion.
Keywords:
Blood-Brain Barrier,
Cerebrum,
neurodegeneration,
in vivo,
hypoperfusion
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Sekaran
H,
Hassan
Z and
Yusof
S
(2016). Regional extravasation of Evans blue dye in the brain across the blood-brain barrier during cerebral hypoperfusion.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00162
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Siti Rafidah Yusof, Universiti Sains Malaysia, Centre for Drug Research, Minden, Penang, Malaysia, SitiRafidah.Yusof@frontiersin.org