PCP modelling cognitive impairment related to schizophrenia in the mouse
-
1
University of Malaya, Faculty of Medicine, Malaysia
-
2
Sheffield Hallam University, Biomedical Research Centre, United Kingdom
-
3
Universiti Teknologi MARA (UiTM), Faculty of Pharmacy, Malaysia
Phencyclidine (PCP) has been successfully used in modelling cognitive impairment related to schizophrenia in the rat. Unfortunately, the model doesn’t work consistently in the mice and confounded by putative drugs interaction in the previous studies. Thus, an optimisation on the PCP regime and doses is a must in establishing a better mouse model of cognitive impairment related to schizophrenia. The present study aims at establishing the model by comparing a range of PCP doses and implementing drug washout period in our PCP regime (7 days injection, i.p., 24 hour washout period); in order to avoid drug interaction effects. The present study is important as many molecular genetic works have been focused in the mouse than in the rat. We used Morris water maze (MWM) to measure the spatial acquisition and long-term memories. In our first experiment, we compared the performance of mice treated with low dose PCP (2 mg/kg) and the vehicle (saline). However, no significant reduction was found in the performance of mice with the PCP in the MWM. This has led us to the second experiment by comparing higher doses of PCP (5 mg/kg, 10 mg/kg, 20 mg/kg) and the vehicle in the independent sample set. We observed a significant reduction in the performance of mice that were treated with all the three doses; however the PCP at 10 mg/kg is the best among the three in giving robust impairment in spatial acquisition memory. Besides, the PCP at 10 mg/kg also impaired the “path efficacy and latency to first entry to the platform zone” in the probe test, indicating an impairment in the long-term memory. Our findings suggested that the presence of washout period is necessary for the PCP regime, and the PCP drug works best at 10 mg/kg in modelling the cognitive impairment.
Keywords:
Cognition,
Schizophrenia,
in vivo,
behavioural assessment,
Phencyclidine
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Zain
M,
Pandy
V,
Reynolds
GP,
Rouhollahi
E,
Wong Feng
W,
Abdul Majed
A and
Mohamed
Z
(2016). PCP modelling cognitive impairment related to schizophrenia in the mouse.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00173
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Mr. Mohd Aizat Zain, University of Malaya, Faculty of Medicine, Kuala Lumpur, Wilayah Persekutuan, Malaysia, aizat03@gmail.com