B-TCE rescues hippocampal neurons from glutamate induced excitotoxicity
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1
Guru Nanak Dev University, Department of Biotechnology, India
Neurons are core components of CNS. Different neurodegenerative diseases like Parkinson’s, Alzheimer’s, Huntington’s and amyotrophic lateral sclerosis are associated with structural and functional loss of neurons. All of these diseases have different initial causes but share a final common destructive pathway such as glutamate-induced excitotoxicity. Glutamate, a major neurotransmitter in CNS plays an important role in excitatory neurotransmission but impaired glutamate homeostasis activates various neurotoxic and excitotoxic cascades involving impaired calcium homeostasis, mitochondrial dysfunction and increased oxidative stress. In traditional practice of medicine, several plants have been reported to possess neuroprotective activity as phytochemicals from these plants play an important role in maintaining chemical balance of the brain. Tinospora cordifolia, one of the most studied medicinal plants has been reported to possess neuroprotective activity in a few neurologic disorders. The present study was carried out to check neuroprotective potential of B-TCE against glutamate-mediated excitotoxicity using primary hippocampal neurons as a model system. Four groups of cells were studied; a contro group, a glutamate treated group, a group treated B-TCE+glutamate and a group treated with B-TCE alone. Changes in cellular morphology, expression of α-Tubulin, NF-kB, MAP-2, GAP-43 and PSA-NCAM & NCAM were studied. Morphology studies showed significant increase in process length in B-TCE+glutamate and B-TCE alone treated cells whereas cells with stunted processes and degenerated morphology were observed in glutamate. Significantly enhanced expression of GAP-43, MAP-2 and NF-kB indicates neuronal growth promotion, differentiation and anti-apoptotic activity of B-TCE. Significant increase in PSA-NCAM & NCAM expression in B-TCE treated groups as compared to glutamate treated group also suggests that it promotes neurite outgrowth and neuronal migration. Thus current study indicates that B-TCE protects the primary hippocampal neuronal cultures from catastrophic consequences of glutamate-induced excitotoxicity. Further detailed study is required to understand underlying mechanism.
Keywords:
Hippocampus,
Glutamate,
in vivo,
excitotoxicity,
natural product
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Sharma
A and
Kaur
G
(2016). B-TCE rescues hippocampal neurons from glutamate induced excitotoxicity.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00204
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Gurcharan Kaur, Guru Nanak Dev University, Department of Biotechnology, Amritsar, PUNJAB, India, kgurcharan.neuro@yahoo.com