Studying the mechanisms controlling cortical neurogenesis with an embryonic stem-cell based model.
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1
Université Libre de Bruxelles, IRIBHM, Belgium
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2
Ghent University Hospital, Department of Neurology, Belgium
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3
Welbio, Belgium
One of the most challenging questions in developmental neurobiology is to understand how neural progenitors/stem cells can generate the diverse repertoire of neuronal subtypes that will form the six layered structure of the cerebral cortex. One mechanism involved is 'temporal patterning', referring to the sequential generation of different sets of neurons over time.
In this project we took advantage of a recently developed in vitro system of corticogenesis based on mouse embryonic stem (ES) cells culture (Gaspard et al., Nature, 2008). In vitro corticogenesis from ES cells recapitulates the major features of neocortex development, in particular the sequential generation of neurons.
Our project consists of using this model to screen for transcription factors involved in neurogenesis in general and in temporal patterning in particular. To this aim, transcription factors that are candidates for shaping neurogenesis are introduced by homologous recombination in an adapted ES cell line that permits their controlled expression by tetracycline. These genetically modified ES cells are subsequently engaged in the corticogenesis process and the impact of these transcription factors on neurogenesis and temporal patterning is determined. Subsequently, transcription factors with an interesting mode of action in vitro are further studied in vivo during mouse embryogenesis using gain and loss of function strategies.
Acknowledgements
Luca Tiberi1* and Jelle van den Ameele1,2*, Jordane Dimidschstein1, Julie Piccirilli1, David Gall3, Adèle Herpoel1, Angéline Bilheu1, Jerome Bonnefont1, Michelina Iacovino4, Michael Kyba4, Tristan Bouschet1, Pierre Vanderhaeghen1,5,6.
* These authors contributed equally to this work.
1 IRIBHM, ULB, Brussels, Belgium. 2 Dept of Neurology, Ghent University Hospital, Ghent, Belgium. 3 Laboratory of Neurophysiology, ULB, Brussels, Belgium. 4 University of Minnesota, USA. 5 Welbio.
Keywords:
Neurogenesis,
Cerebral Cortex,
Embryonic Stem Cells,
Developmental Biology,
mouse model
Conference:
Belgian Brain Council, Liège, Belgium, 27 Oct - 27 Oct, 2012.
Presentation Type:
Poster Presentation
Topic:
Other basic/clinical neurosciences topic
Citation:
Van Den Ameele
J,
Tiberi
L and
Vanderhaeghen
P
(2012). Studying the mechanisms controlling cortical neurogenesis with an embryonic stem-cell based model..
Conference Abstract:
Belgian Brain Council.
doi: 10.3389/conf.fnhum.2012.210.00113
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Received:
27 Aug 2012;
Published Online:
12 Sep 2012.
*
Correspondence:
Dr. Jelle Van Den Ameele, Université Libre de Bruxelles, IRIBHM, Bruxelles, Belgium, jelle.vandenameele@ugent.be