Visual evoked potentials in Lurcher mutant mice: infuence of retinal degeneration as an independent pathogeny
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1
Charles University, Faculty of Medicine Pilsen, Dept. of Pathophysiology, Czechia
Interaction between inborn retinal degeneration and visual evoked potentials has been studied using Lurcher mutant mice (LMM). LMM represent a natural model of genetically determined olivocerebellar degeneration (OCD) which is caused by a semidominant mutation (Grid2Lc) of glutamate receptor gene (GluRδ2). Heterozygotes (+/Lc) suffer from excitotoxic apoptosis of Purkinje cells and secondary loss of granule cells and inferior olivary neurons. Main consequences are a cerebellar ataxy syndrome and an impairment of some cognitive functions. We examined a C3H strain of LMM, in which a retinal degeneration determined by autosomal recessive form of Pde6brd1 gene mutation (rd1 type in older papers) independently occurs. Thus, there are exist four possible phenotypes of animal: „blind“ with or without OCD and „seeing“ with or without OCD. The aim of this study was to compare an ability to create of visual evoked potentials (VEP) with histological control in selected LMM. Cortical VEP were registered unilaterally in primary visual cortex, possible retinal degeneration was detected histologically using a hematoxylin-eosin staining.
Results of VEP investigation suggest similarities between wild type mice (healthy controls) and +/Lc of C3H strain; some of them revealed a gentle shift to longer latencies and decrease of amplitudes. In these cases, histological controls of retinal slices showed an absence of outer nuclear layer. But generally, the ability to generate of VEP in „blind“ animals was preserved. Our results show that retinal degeneration (confirmed by the histological examination) and cortical VEP does not correlate. Possible explanation of this important phenomenon could be in functional neuronal plasticity in retina.
Acknowledgements
This research was supported by the Charles University
Research Fund (project number P36)
Keywords:
Retinal Degeneration,
Lurcher mutant mice,
Olivocerebellar pathology,
Neuronal Plasticity,
visual evoked potential (VEP)
Conference:
SAN2016 Meeting, Corfu, Greece, 6 Oct - 9 Oct, 2016.
Presentation Type:
Poster Presentation in SAN2016 Conference
Topic:
Posters
Citation:
Barcal
J,
Petránková
Z,
Voller
J and
Vožeh
F
(2016). Visual evoked potentials in Lurcher mutant mice: infuence of retinal degeneration as an independent pathogeny.
Conference Abstract:
SAN2016 Meeting.
doi: 10.3389/conf.fnhum.2016.220.00067
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Received:
29 Jul 2016;
Published Online:
01 Aug 2016.
*
Correspondence:
MD, PhD. Jan Barcal, Charles University, Faculty of Medicine Pilsen, Dept. of Pathophysiology, Pilsen, 301 00, Czechia, jan.barcal@lfp.cuni.cz