Brain-wide atlas of amyloid-beta distribution in transgenic amyloid precursor protein mouse model of Alzheimer’s disease
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1
University of Oslo, Department of Anatomy, Institute of Basic Medical Sciences, Norway
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2
University of Oslo, Department of Pharmacology, Norway
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3
Oslo University Hospital, Norway
Genetic animal models are powerful tools for preclinical experimental investigations of pathogenic processes, diagnostic markers and identification and evaluation of novel interventional approaches. Numerous genetic animal models reflect important characteristics of different diseases, but none reproduce the full spectrum of pathological changes seen in human patients. The selection of appropriate models is a considerable challenge and requires knowledge about the genetic background, behavioral alterations and neuropathological phenotype. For neurodegenerative diseases, such as Alzheimer’s disease, detailed information about the morphology, spatial distribution and amount of key pathological hallmarks, such as aggregation of amyloid-β plaques in the brain. A considerable general challenge for the field is that most studies are typically restricted to selected brain regions, and document their observations journal figures as representative selected examples. This poses an important limitation to the knowledge that can be gained from morphological phenotyping studies, and there is a growing awareness about the need for new resources allowing researchers to share large amounts neuroanatomical data image data describing important features of genetic disease models. We have developed a database infrastructure for efficient dissemination of serial microscopic image data from rodent brains, and here present an atlas showing immuno-labeled amyloid-β plaques in 12 month old female and male transgenic mice carrying the Arctic (E693G) and Swedish (KM670/6701NL) amyloid precursor protein mutations. Our atlas system (http://www.rbwb.org/, see tg-ArcSwe atlas) allows online inspection of the histological characteristics and spatial distribution of immuno-labeled amyloid plaque. We demonstrate how the image collection facilitates brain-wide characterization and quantitative image analysis of labeled neuropathological features. We argue that accumulation of comprehensive morphological data on multiple animal models in digital brain atlas systems will allow better characterization and more efficient selection of animal models for future resource-demanding interventional investigations.
Keywords:
digital atlasing,
Alzheimer Disease,
Amyloid beta,
genetic animal models,
Immunohistochemistry
Conference:
Neuroinformatics 2013, Stockholm, Sweden, 27 Aug - 29 Aug, 2013.
Presentation Type:
Poster
Topic:
Digital atlasing
Citation:
Lillehaug
S,
Syverstad
GH,
Nilsson
LN,
Torp
R,
Bjaalie
JG and
Leergaard
TB
(2013). Brain-wide atlas of amyloid-beta distribution in transgenic amyloid precursor protein mouse model of Alzheimer’s disease.
Front. Neuroinform.
Conference Abstract:
Neuroinformatics 2013.
doi: 10.3389/conf.fninf.2013.09.00002
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Received:
05 Jun 2013;
Published Online:
11 Jul 2013.
*
Correspondence:
Mr. Sveinung Lillehaug, University of Oslo, Department of Anatomy, Institute of Basic Medical Sciences, Oslo, Norway, sveinung.lillehaugj.g.bjaalie@medisin.uio.no