Large scale whole brain mapping of inputs to the main olfactory bulb
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1
Salk Institute for Biological Studies, United States
An important prerequisite to understanding how neural circuits generate behavior is to understand the structure of those circuits, including the connectivity between the neurons. In sensory systems, a key component of that connectivity is the top down projections from higher cortical regions to primary sensory areas. For example, in mammals, projections from olfactory cortical regions, including piriform cortex, represent a major input to the inhibitory granule neurons in the main olfactory bulb. Inhibitory granule cells play a central role in how odor information is transformed and represented by the principal neurons of the bulb, the mitral cells. Projections from higher odor areas including the accessory olfactory nucleus and the piriform cortex are known to impact the firing of both the granule cells and the mitral cells, highlighting their importance in shaping how odors are represented. However, little is know about the organization of top-down inputs to these inhibitory cells in large part because of the experimental and computational challenges of single cell mapping across a whole brain. To provide a complete portrait of the monosynaptic inputs to inhibitory cells in the main olfactory bulb, we employed the rabies virus trans-synaptic tracing technique and developed an imaging platform that allowed us to visual every single labeled cell across the entire mouse brain. Labeling spatially distinct populations of inhibitory granule cells in the bulb with the modified rabies allowed us to trace the patterns of innervation from a number of higher processing areas, including the accessory olfactory nucleus and the olfactory cortex. From these injections, we were able to reconstruct, identify and classify the presynaptic partners to inhibitory cells in the bulb across the entire mouse brain at single cell resolution. Our data reveal the complex patterns of innervation to the inhibitory neurons of the bulb, and provide a map of the spatial organization of feedback projections for higher brain areas to their lower processing counterparts. By integrating the connectivity maps of inputs into the bulb on the scale of the whole mouse brain with models of activity patterns within the bulb, we hope to provide insight into how cortical feedback may play a role in shaping the representations of odor information.
Keywords:
whole brain imaging,
large scale connectivity maps,
Olfactory Bulb,
granule cells,
piriform cortex,
accessory olfactory nuclues,
top-down processing,
top down processing
Conference:
Neuroinformatics 2013, Stockholm, Sweden, 27 Aug - 29 Aug, 2013.
Presentation Type:
Oral presentation
Topic:
Large scale modeling
Citation:
Padmanabhan
K,
Osakada
F,
Tarabrina
A,
Kizer
E,
Gage
FH and
Callaway
EM
(2013). Large scale whole brain mapping of inputs to the main olfactory bulb.
Front. Neuroinform.
Conference Abstract:
Neuroinformatics 2013.
doi: 10.3389/conf.fninf.2013.09.00065
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Received:
29 Apr 2013;
Published Online:
11 Jul 2013.
*
Correspondence:
Dr. Krishnan Padmanabhan, Salk Institute for Biological Studies, La Jolla, CA, 92037, United States, krishnan@salk.edu