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Image segmentation based on Frequency domain operation (FFT) and plugin implementation for ImageJ

Víctor J. García Fernández1* and Dimiter Prodanov2
  • 1 IMEC, Belgium
  • 2 IMEC, EHS, Belgium

In order to better measure cell’s response to different chemical or mechanical stimuli robust quantification of the cell spatio-temporal organization is required. Accurate identification of individual cellular phenotypes can be obtained by combining automated microscopic acquisition with extensive morphological feature extraction and data mining strategies. The aim of the present work is to implement filter banks allowing for measurement of scale-invariant features which will be used for downstream image classification operations. A generic way of segmenting images is by using multi-scale approaches, such as wavelet filter banks or scale spaces. Fourier domain filtering encodes image information in a distributed manner that allows manipulation of the global information content of the image scaling the performance of such filtering operations. The poster and the software demo will present a generic framework for Fourier domain filtering made available as a plugin to the popular public domain image processing software ImageJ. Filter implementations include Gaussian, Laplacian of Gaussian and Fractional Laplacian operators. We could successfully apply this plugin for example in segmentation of histological images.

Acknowledgements

The work has been supported in part by a grant from Research Fund - Flanders (FWO), contract number 0880.212.840.

References

Prodanov D (2013). Open source image analysis software toolboxes for microscopic applications. Front. Neuroinform. Conference Abstract: Imaging the brain at different scales: How to integrate multi-scale structural information?. doi: 10.3389/conf.fninf.2013.10.00005

Lindeberg, T. (1994). Scale-space theory: A basic tool for analysing structures at different scales. J App Stat. 21 (2): 224–270.

Mols K, Prodanov D and Bonin V (2013). Scale-space based segmentation of cells in functional two-photon in vivo images. Front. Neuroinform. Conference Abstract: Imaging the brain at different scales: How to integrate multi-scale structural information?. doi: 10.3389/conf.fninf.2013.10.00033

Keywords: Image Processing, Computer-Assisted, FFT, filtering, Cell segmentation, ImageJ

Conference: Second Belgian Neuroinformatics Congress, Leuven, Belgium, 4 Dec - 4 Dec, 2015.

Presentation Type: Poster Presentation

Topic: Brain Imaging

Citation: García Fernández VJ and Prodanov D (2015). Image segmentation based on Frequency domain operation (FFT) and plugin implementation for ImageJ. Front. Neuroinform. Conference Abstract: Second Belgian Neuroinformatics Congress. doi: 10.3389/conf.fninf.2015.19.00010

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Received: 31 Oct 2015; Published Online: 17 Nov 2015.

* Correspondence: Mr. Víctor J García Fernández, IMEC, Leuven, Belgium, victorjgf@gmail.com