Measurement of Magnetic Field of brain activities in rat using micro SQUID
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1
Osaka City University Graduate School of Medicine, Department of Neurosurgery, Japan
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2
Kanazawa Institute of Technology, Japan
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3
Osaka City University Graduate School of Medicine, Department of Physiology, Japan
Objective: Magnetoencephalography　(MEG) is used to study brain function in humans, but the limited resolution and sensitivity of conventional instruments have precluded the use of MEG to study neuronal function in rodents. We demonstrate that micro MEG system developed for use with small animals, can be used to detect assess neuronal activity in conscious rodent brain and epilepsy-model rat. Methods and Results: We used a micro 9-channel magnetometer consisting of a 3 × 3 matrix of superconducting quantum interference device (SQUID) with its integrated base of 2.5 mm × 2.5 mm. In the first step, we to measure the visual evoked magnetic field (VEF) and auditory evoked field (AEF) of rats. We obtained the VEF wave with 60-80ms peak by the white LED flashing stimulation and the AEF wave with 10-20ms peak by the tone and burst stimulation. VEFs were measured at a significant level of reproducibility in 4 out of the 5 rats, and .AEFs done in 3 out of the 5 rats. In the second step, we generated the epilepsy ?mode rats which were intraperitoneally injected kainic acid. Those rats were found to have severe epileptic seizures. We simultenaously measured EEG and MEG. We found epileptogenic spikes for EEG, but it was difficult to detect small magnetic abnormal signals. Conclusions: These studies demonstrate that micro MEG can be used for serial assessment of neuronal function of individual, live animals with a minimal degree of invasiveness, has the potential for use in the study of brain function and plasticity. If the MEG system can detect epileptogenic spikes, it will perhaps make a great contribution to understanding the mechanism by which epilepsy develops.
Conference:
Biomag 2010 - 17th International Conference on Biomagnetism , Dubrovnik, Croatia, 28 Mar - 1 Apr, 2010.
Presentation Type:
Poster Presentation
Topic:
Epilepsy
Citation:
Tsuyuguchi
N,
Adachi
Y,
Uda
T,
Shigihara
Y,
Ohata
K and
Uehara
G
(2010). Measurement of Magnetic Field of brain activities in rat using micro SQUID.
Front. Neurosci.
Conference Abstract:
Biomag 2010 - 17th International Conference on Biomagnetism .
doi: 10.3389/conf.fnins.2010.06.00355
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Received:
07 Apr 2010;
Published Online:
07 Apr 2010.
*
Correspondence:
Naohiro Tsuyuguchi, Osaka City University Graduate School of Medicine, Department of Neurosurgery, Osaka, Japan, tnaohiro@aol.com