A light- and electronmicroscopic immunocytochemical, and pharmacological characterization of the developing 5-HTergic system in the buccal mass
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1
Balaton Limnological Research Institute, Hungarian Academy of Sciences, Comparative Neurobiology Research Group, Department of Experimental Zoology, Hungary
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2
Russian Academy of Science, Institute of Developmental Biology, Russia
In this study, we investigated the 5-HT-immunoreactive (5-HT-IR) innervation pattern and the physiological-pharmacological characteristics of the 5-HTergic system in the buccal mass of the developing pond snail Lymnaea stagnalis, which is responsible for the execution of feeding (radula protraction). According to laser confocal microscopy applied after 5-HT and phalloidin immunocytochemistry, 5-HT-IR elements could not be seen in the buccal mass following the metamorphosis (E60%, E70%) but in the CNS and other peripherial regions (foot, body wall). From E80% embryonic stage a fast maturation was characteristic for the 5-HT-IR innervation forming thin varicose processes in the buccal muscle. By hatching, 5-HT-IR processes established a rich ramification in and among the longitudinal and circular muscle bundles. The 5-HT-IR fibers showed a dense innervation pattern by the mid-juvenile stage (P3), when their organization already resembled that observed in adults. At ultrastructural level, TEM investigations revealed close membrane contacts between agranular and granular vesicule containing varicosities and muscle cells in hatchlings and early juveniles. Long 5-HT-IR axon processes and unlabeled varicosities containing electron dense granules were seen nearby the muscle cells in the late embryonic, juvenile and adult buccal mass. Physiological-pharmacological experiments suggested that an inhibitory 5-HT1-like and a stimulatory 5-HT6-like receptor are involved in the regulation of feeding activity (radula protraction). The 5-HT6-like receptor was shown to be positively coupled to an adenylate cyclase (cAMP) second messenger system. It is concluded that 5-HT is a transmitter candidate at neuromuscular level in the peripherial feeding system (buccal mass) of Lymnaea and plays a relevant role in the early functional maturation of the buccal mass.
Support: OTKA grant, No. 78224, to K. E. and HAS-RAS exchange program.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Development
Citation:
Balog
GM,
Voronezhskaya
EE,
Elekes
K and
Hiripi
L
(2010). A light- and electronmicroscopic immunocytochemical, and pharmacological characterization of the developing 5-HTergic system in the buccal mass.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00001
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Received:
14 Apr 2010;
Published Online:
14 Apr 2010.
*
Correspondence:
Gábor M Balog, Balaton Limnological Research Institute, Hungarian Academy of Sciences, Comparative Neurobiology Research Group, Department of Experimental Zoology, Tihany, Hungary, balogg@tres.blki.hu