Selective adhesion and long-term culturing of radial glia-like neural stem cells on a synthetic peptide-conjugate
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1
Hungarian Academy of Science, Institute of Experimental Medicine, Hungary
In vitro propagation of homogeneous populations of neural stem/progenitor cells bear great significance. Here we report that selective adhesion of neural stem cells to a novel synthetic AK-cyclo[RGDfC] polypeptide allows a unique, one-step isolation of radial glia-like (RG) cells from fetal or adult rodent brain. On AK-cyclo[RGDfC]-covered surfaces, radial glia-like neural stem/progenitor cells can be maintained and propagated in chemically defined, xeno-free culture conditions. In the presence of EGF and insulin, as only growth factor supplements, RG cells display radial glia-specific features including nestin, RC2 immunoreactivity and Pax6, Sox2, Blbp, Glast gene expression. In response to EGF withdrawal, RG cells give rise to neurons regardless of embryonic or adult origin. In differentiating cultures, GABAergic and glutamatergic neurons developed, but monoaminergic neurotransmitter phenotypes were not formed. Besides neurons, RG cells can differentiate to astrocytes or oligodendrocytes as well.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Development
Citation:
Markó
K,
Kohidi
T,
Jelitai
M,
Hádinger
N and
Madarász
E
(2010). Selective adhesion and long-term culturing of radial glia-like neural stem cells on a synthetic peptide-conjugate.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00015
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Received:
16 Apr 2010;
Published Online:
16 Apr 2010.
*
Correspondence:
Károly Markó, Hungarian Academy of Science, Institute of Experimental Medicine, Budapest, Hungary, marko@koki.hu