Bone Marrow Derived Non-Reactive Astrocytes in the Mouse Brain after Permanent Middle Cerebral Artery Occlusion
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1
Semmelweis University, Institute of Anatomy, Hitology and Embriology, Hungary
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2
NINDS, National Institutes of Health, United States
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3
NIMH, National Institutes of Health, United States
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4
NIDCR, National Institutes of Health, United States
We investigated the effect of permanent unilateral middle cerebral artery occlusion (PMCAO) on the generation of bone marrow (BM) derived astrocytes in female mice previously transplanted with eGFP-expressing BM from male donors. In addition to an untreated PMCAO group, one group of mice further received intracerebral infusion of transforming growth factor-alpha (TGFα) treatment, that resulted in a decrease in the size of the infarct. We found specific type of astrocytes of BM origin two month after PMCAO in the side of the injury and near the lesion. These astrocytes did not express glial fibrillary acidic protein (GFAP) as seen by conventional fluorescence immunostaining, however, GFAP was strongly detectable by tyramide signal amplification (TSA). These BM derived astrocytes did not proliferate and did not contribute to the glial scar formation, unlike the endogenous reactive astrocytes. TGFα resulted in an increase of BM derived astrocytes, but had no effect on their distribution. Interestingly, exclusively by TSA staining we found that endogenous astrocytes displaying an identical morphology were also present in control brains, as well as in both sides of the brains of ischemic mice.
Our data demonstrate that a subpopulation of astrocytes that expresses low levels of GFAP originates from transplanted BM cells in the ischaemic brain. We believe these cells represent a subpopulation of astrocytes that were earlier considered to be GFAP negative. We suggest that these astrocytes might play an important structural role in the regeneration process following ischaemic injury, further strengthening the idea that BM cells might potentially be used as vectors to deliver survival factors to the injured CNS.
Supported by, ETT 495/09. Tóth ZE is supported by the Bolyai fellowship.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Disorders of the nervous system
Citation:
Tóth
Z,
Leker
RR,
Tal
S,
Bratincsak
A,
Szalayova
I,
Key
S,
Cassiani-Ingoni
R and
Mezey
E
(2010). Bone Marrow Derived Non-Reactive Astrocytes in the Mouse Brain after Permanent Middle Cerebral Artery Occlusion.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00068
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Received:
21 Apr 2010;
Published Online:
21 Apr 2010.
*
Correspondence:
Zsuzsanna Tóth, Semmelweis University, Institute of Anatomy, Hitology and Embriology, Budapest, Hungary, tothzs@ana.sote.hu