Ion currents and gap junction coupling in isolated radial glial cells
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1
Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary
According to the accepted view, cell-physiological properties of stem and progenitor cells contribute to the maintenance of a non-differentiated status even in a highly differentiated environment, but help to respond to specific differentiation inducing signals. The cellular “excitability” and responsiveness to environmental stimuli are strongly influenced by the local changes in the intracellular distribution of ions. Changes in the expression and in the functional properties of ion channels play deterministic role in neuronal commitment and neuronal differentiation.
In the current work, the characteristics of selectively isolated radial glial cells from embryonic or adult nerve tissue- were studied using dye spreading and patch-clamp techniques.
Radial glial cells are less gap junction coupled than the neural stem cells and the rate of gap junction coupling changes dynamically. According to the level of gap junction coupling radial glial cells display passive conductance. Delayed-rectifying outward potassium current was expressed almost in all isolated radial glial cell, however the pharmacological properties of the current changed significantly with development. In cells with neuronal morphology voltage dependent sodium current was recorded. The characterization of voltage-dependent ion currents and their developmental appearance may reveal some regulatory roles of ion channel functions in the maintenance of the progenitor stage.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Cellular neuroscience
Citation:
Jelitai
M,
Markó
K,
Hádinger
N and
Madarász
E
(2010). Ion currents and gap junction coupling in isolated radial glial cells.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00071
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Received:
21 Apr 2010;
Published Online:
21 Apr 2010.
*
Correspondence:
Márta Jelitai, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, jelitai@koki.hu