Intracerebroventricular injection of a parathyroid hormone 2 receptor antagonist abolishes suckling induced prolactin release
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1
Hungarian Academy of Science, Neuromorphological and Neuroendocrine Research Laboratory, Department Anatomy, Histology and Embryology, Hungary
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2
Semmelweis University, Hungary
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3
National Institute of Mental Health, Section on Fundamental Neuroscience, United States
Tuberoinfundibular peptide of 39 residues (TIP39) and the parathyroid hormone 2 receptor (PTH2R) constitute a peptide-receptor neuromodulator system implicated in neuroendocrine, anxiety-related and nociceptive functions. TIP39 neurons are restricted to 3 brain regions: the medial subparafascicular area (SPF) and the posterior intralaminar complex of the thalamus (PIL), and the medial paralemniscal nucleus (MPL) in the lateral pons. By using real-time RT-PCR, in situ hybridization histochemistry, and immunohistochemistry, we found that TIP39 mRNA and peptide are markedly elevated in the PIL of lactating dams compared to the PIL of mothers separated from their pups. TIP39 neurons in the PIL project to the arcuate and periventricular nuclei of the hypothalamus where the PTH2R is also concentrated. To investigate a potential function of TIP39 in mother rats, they were separated from their pups for 4 hours on postpartum day 9, and a PTH2R antagonist was injected via cannulae placed into the lateral ventricle 5 min before the pups were returned to their mothers. Blood samples were taken via cannulae in the right jugular vein of mothers prior to separation, at the end of the 4 hours separation period, and then 5, 15, 30 and 60 min after the suckling stimulus was initiated. The PTH2R antagonist almost completely blocked suckling-induced elevation of plasma prolactin. In contrast, injection of the PTH2R antagonist had no effect in pup retrieval test indicating that TIP39 might not influence maternal behaviors. Therefore, our results suggest that TIP39-PTH2R signaling at the level of the hypothalamus is activated during suckling stimulus and possibly inhibits dopaminergic neurons that control prolactin secretion in the hypothalamo-pituitary system.
The work was supported by the Bolyai Fellowship of the HAS, NKTH-OTKA K67646 and K68170, NFM-OTKA NNF78219 and OTKA NK72929 Research Grants and the NIMH Intramural Program.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Homeostatic and neuroendocrine systems
Citation:
Cservenák
M,
Bodnár
I,
Usdin
TB,
Palkovits
M,
Nagy
G and
Dobolyi
A
(2010). Intracerebroventricular injection of a parathyroid hormone 2 receptor antagonist abolishes suckling induced prolactin release.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00081
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Received:
22 Apr 2010;
Published Online:
22 Apr 2010.
*
Correspondence:
Melinda Cservenák, Hungarian Academy of Science, Neuromorphological and Neuroendocrine Research Laboratory, Department Anatomy, Histology and Embryology, Budapest, Hungary, csermel@gmail.com