Differential Regulation of Hypothalamic Neuropeptide Y hnRNA and mRNA during Psychological Stress and Insulin-induced Hypoglycemia
-
1
HAS, Institute of Experimental Medicine, Hungary
Many signals reflecting energy balance and stress are integrated at the hypothalamic orexigenic NPY neurons. To determine transcriptional changes of the NPY gene in response to stress challenges, we followed and compared the expression of heteronuclear (hn)- and messenger (m)RNA levels by in situ hybridization histochemistry and by Real-Time PCR assays in mice following insulin-induced hypoglycemia and restraint. Hypoglycemia in fasted mice resulted in a rapid increase of NPY hnRNA that peaked at 1 hour, declined thereafter by 2-4 hours after insulin injection and run parallel to that of NPY mRNA. Throughout the time course examined, NPY expressing cells in the medial-basal hypothalamus remained overwhelmingly localized to the arcuate nucleus. Following restraint stress, NPY mRNA signal was increased, however hnRNA levels decreased, suggesting increased stability of mature NPY mRNA. These results highlight rapid changes and differential regulation of NPY expression in response to metabolic and stress challenges.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Homeostatic and neuroendocrine systems
Citation:
Ferenczi
S
(2010). Differential Regulation of Hypothalamic Neuropeptide Y hnRNA and mRNA during Psychological Stress and Insulin-induced Hypoglycemia.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00086
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
22 Apr 2010;
Published Online:
22 Apr 2010.
*
Correspondence:
Szilamér Ferenczi, HAS, Institute of Experimental Medicine, Budapest, Hungary, ferenczi@koki.hu