Effects of local noradrenaline depletion in the medial prefrontal cortex and body weight regulation
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1
University of Pécs, Medical School, Institute of Physiology and Neurophysiology Research Group of the Hungarian Academy of Sciences (HAS), Hungary
In vivo microdialysis studies showed that in the medial prefrontal cortex (mPFC) dopamine (DA) and noradrenaline (NA) levels were enhanced during feeding. Our earlier results showed that DA terminals of the mPFC are involved in the regulation of body weight. The aim of the present experiments was to study the effects of lesions of NA terminals in the mPFC on the regulation of body weight. In loco injection of 6-hydroxy-DOPA (6-OHDOPA) was applied. In other group, a potent DA uptake inhibitor, GBR12935 was injected before the administration of neurotoxin 6-hydroxy-dopamine (6-OHDA), which acts on both DA and NA terminals. The control groups were injected with vehicle or GBR12935. Daily food and water intake and body weight were measured to the nearest g and ml, respectively. Regulatory deficits after 24 h food and water deprivation were also examined. DA and NA levels were determined in vitro from micro punched samples of the mPFC. Only transient body weight decrease was found in groups with neurochemical lesions. There was no difference among groups in 24 h food and water intake and 1 h food and water intake after 24 h deprivations. Lesions of NA terminals by 6-OHDOPA depleted the NA level only by 21 % [p<0.05]. 6-OHDOPA lesion caused elevated DA levels by 122 % [p<0.01]. Concentration of NA was reduced by 43 % [p<0.01] due to GBR 12935 + 6-OHDA lesion. The DA levels were elevated in these cases by 195 % [p<0.01]. In summary, NA lesion was followed by enhanced DA activity in the mPFC but, only a transient body weight decrease was detected.
This study was supported by NKTH-OTKA K68431 and by the Hungarian Academy of Sciences.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Homeostatic and neuroendocrine systems
Citation:
Gálosi
R,
Tóth
A,
Petykó
Z and
Lénárd
L
(2010). Effects of local noradrenaline depletion in the medial prefrontal cortex and body weight regulation.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00088
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Received:
22 Apr 2010;
Published Online:
22 Apr 2010.
*
Correspondence:
Rita Gálosi, University of Pécs, Medical School, Institute of Physiology and Neurophysiology Research Group of the Hungarian Academy of Sciences (HAS), Pécs, Hungary, rita.galosi@aok.pte.hu