Induction of FosB/ΔFOSB int he brain stress system-related structures during morphine dependence and withdrawal
-
1
University School of Medicine, Department of Pharmacology, Spain
-
2
Institute of Experimental Medicine, Laboratory of Molecular Neuroendocrinology, Hungary
Repeated exposure to drugs of abuse causes cellular adaptations in specific neuronal populations that ultimately lead to a state of addiction. It has been suggested that changes in gene expression patterns are important mechanism by which chronic exposure to a drug of abuse causes long-lasting alterations in the brain, which underlie neuroendocrine, autonomic and behavioural abnormalities. Repeated administration of drug of abuse increases ΔFosB levels in the brain reward system ie. the nucleus accumbens (NAc). The present study was designed to evaluate the possible modifications in ΔFosB expression in both hypothalamic and extrahypothalamic brain CRF systems during morphine dependence and withdrawal. Rats were made dependent on morphine for seven days then injected with saline or naloxone. Using immunohistochemical procedures, the expression of FosB/ΔFosB in CRF-positive neurons was measured in the central amygdala (CeA), bed nucleus of the stria terminalis (BNST) and hypothalamic paraventricular nucleus (PVN)] in morphine dependent rats and after morphine withdrawal. FosB/ΔFosB was induced after chronic morphine administration in the CRF-containing neurons of the parvocellular part of the PVN. In addition, in morphine-withdrawn rats there was an induction of FosB/ΔFosB in CRF-positive cells in the PVN, CeA and BNST. The presented data indicate that neuroadaptation to addictive substances, observed as accumulation of FosB/ΔFosB transcription factor, is not limited to the reward circuits of the brain but manifest in other brain regions which have been proposed to be directly related to addiction.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Cellular neuroscience
Citation:
Milanés
VM,
Martin
F,
Núñez
C,
Földes
A,
Laorden
LM and
Kovács
KJ
(2010). Induction of FosB/ΔFOSB int he brain stress system-related structures during morphine dependence and withdrawal.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00092
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
22 Apr 2010;
Published Online:
22 Apr 2010.
*
Correspondence:
Victoria M Milanés, University School of Medicine, Department of Pharmacology, Murcia, Spain, milanes@um.es