Unitary volume transmission by neurogliaform cells targets neural and glial elements in the neocortical microcircuit
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1
University of Szeged, Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, Hungary
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2
University of California, United States
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3
Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary
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4
University of Szeged, Department of Neurosurgery, Hungary
A distinctive feature of neurogliaform cells among cortical interneurons is the dense axonal arborization in which presynaptic boutons on the same or neighboring collaterals can be found a couple micrometers from one another. GABA can activate receptors located up to several micrometers from the release site. We hypothesized that release of GABA from neurogliaform cells might reach the majority of receptors expressed in the tissue intermingled by the axonal cloud. Using multiple whole cell patch clamp recordings, we recently showed that that individual neurogliaform cells release GABA in concentrations sufficient for volume transmission within the axonal cloud. Thus, that neurogliaform cells do not require synapses to produce inhibitory responses in the overwhelming majority of nearby neurons. Neurogliaform cells suppress connections between other neurons acting on presynaptic terminals that do not receive synapses at all in the cerebral cortex. To analyze possible impact of neurogliaform cells on neighboring astrocytes, we used two-photon imaging in layers 1-3 of brain slices taken from the somatosensory cortex of young rats. Cells were loaded with the fluorescent Ca2+ indicator Oregon Green BAPTA-1 (120 μM), and we used Alexa-594 (40 μM) to label astrocytic processes. Action potentials in single neurogliaform cells synchronously triggered intracellular Ca+ responses in several neighboring astrocytes within the axonal arborization of the neurogliaform cell with a latency of several seconds.
In line with our recent experiments showing nonsynaptic effects of individual neurogliaform cells on neurons, these data suggest that unitary volume transmission by neurogliaform cells regulates both glial and neural elements of the cortical microcircuit.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Cellular neuroscience
Citation:
Oláh
S,
Füle
M,
Komlósi
G,
Báldi
R,
Varga
C,
Rózsa
B,
Barczó
P and
Tamás
G
(2010). Unitary volume transmission by neurogliaform cells targets neural and glial elements in the neocortical microcircuit.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00115
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Received:
23 Apr 2010;
Published Online:
23 Apr 2010.
*
Correspondence:
Szabolcs Oláh, University of Szeged, Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, Szeged, Hungary, olah.szabolcs.neuro@gmail.com