Firing activity of glycinergic and non-glycinergic neurons in PnO during ketamine-xylazine slow oscillation
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1
Institute of Experimental Medicine, Hungary
The ascending activating system arising from the brainstem is responsible for switching thalamocortical cells to tonic mode by exerting depolarizing actions on them. However in the midbrain reticular core (nucleus pontis oralis - PnO) a great population of GABAergic cells coexpress glycine as inhibitory transmitter as revealed in the glycine transporter type 2 (GlyT2)-EGFP transgenic mouse and these inhibitory cells selectively innervate the intralaminar and midline thalamic nuclei. The GlyT2-positive component consists of the 50 % of this pathway. In the present study our aim was to characterize the in vivo firing pattern of the GlyT2-EGFP expressing and non-expressing neurons of the above PnO-thalamic pathway in anesthetized mice.
In vivo juxtacellular recording and labeling was used to characterize the firing activity and identify PnO cells (n=8). Concurrently the local field potential (LFP) in the prefrontal cortex was recorded and a stimulating electrode was placed in the intralaminar thalamus. The recorded cells showed heterogenous firing properties. All of the glycinergic neurons but only some of the non-glycinergic ones fired rhythmic clusters of action potentials at 50-90 Hz interspersed with high frequency doublets. Clusters occurred in synchrony with the cortical Up states, and the rhythmic activity was disrupted by cortical inactivation. Certain cells could be antidromicaly stimulated from the thalamus with very short latency that confirms the direct connection between the two regions. We have also found a conspicuous activation-inhibition pattern for almost all cells following thalamic stimulation that depended on the stimulation frequency and involves most probably local inhibitory and cortical excitatory mechanisms.
Our preliminary results foreshadow the fine-tuned connection between cortex and the brainstem and raise the possibility of glycinergic-GABAergic pathway having an important role in the regulation of sleep-wake cycle.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Cellular neuroscience
Citation:
Plattner
V,
Bokor
H,
Giber
K and
Acsády
L
(2010). Firing activity of glycinergic and non-glycinergic neurons in PnO during ketamine-xylazine slow oscillation.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00119
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Received:
28 Apr 2010;
Published Online:
28 Apr 2010.
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Correspondence:
Viktor Plattner, Institute of Experimental Medicine, Budapest, Hungary, pviktor@koki.hu